Background： At present, a diagnostic tool with high specificity for impaired endometrial receptivity, which may lead to implantation failure, remains to be developed. We aimed to assess the different ...endometrial microRNA （miRNA） signatures for impaired endometrial receptivity by microarray analysis. Methods： A total of 12 repeated implantation failure （RIF） patients and I0 infertile patients, who conceived and delivered after one embryo transfer attempt, were recruited as RIF and control groups, respectively. Endometrial specimens from the window of implantation （WOI） were collected from these two groups. MiRNA microarray was conducted on seven and five samples from the RIF and control groups, respectively. Comparative, functional, and network analyses were performed for the microarray results. Quantitative real-time polymerase chain reaction （PCR） was performed on other samples to validate the expression of specific miRNAs. Results： Compared with those in the control group, the expression levels of 105 miRNAs in the RIF group were found to be significantly up- or down-regulated （at least 2-fold） by microarray analysis. The most relevant miRNA functional sets of these dysregulated miRNAs were miR-30 family, human embryonic stern cell regulation, epithelial-mesenchymal transition, and miRNA tumor suppressors by tool for annotations ofmicroRNA analysis. Network regulatory analysis found 176 miRNA-mRNA interactions, and the top 3 core miRNAs were has-miR-4668-5p, has-miR-429, and has-miR-5088. Expression levels of the 18 selected miRNAs in new samples by real-time PCR were found to be regulated with the same trend, as the result ofmicroarray analysis. Conclusions： There is a significant different expression of certain miRNAs in the WOI endometrium for RIF patients. These miRNAs may contribute to impaired endometrial receptivity.
Bridging chiral p‐tert‐butylcalix4arenes (p‐t‐Bu‐BCC's) with different N‐substituted carbamoyl bridge‐substituents (N,N‐dimethylcarbamoyl, N,N‐diethylcarbamoyl and morpholinocarbonyl) were ...successfully prepared through anionic ortho‐Fries rearrangement from mono‐O‐carbamates of 1,3‐dipropyl‐p‐tert‐butylcalix4arene in 65–75% yield. In addition, p‐t‐Bu‐BCC with two N,N‐dimethylcarbamoyl bridge‐substituents was produced by this method from mono‐O‐carbamate of p‐t‐Bu‐BCC with one N,N‐dimethylcarbamoyl bridge‐substituent in 71% yield. However, the synthesis of p‐t‐Bu‐BCC with additional carbamoyl bridge‐substituents using this method could not be attempted, as the required rearrangement precursor failed to be synthesized. Finally, the racemic p‐t‐Bu‐BCC with morpholinocarbonyl bridge‐substituent was optically resolved into a pair of enantiomers, whose absolute configurations were determined through ROESY analysis and ECD comparison.
Bridging chiral p‐tert‐butylcalix4arenes (BCC's) with different N‐substituted carbamoyl bridge‐substituents (N,N‐dimethylcarbamoyl, N,N‐diethylcarbamoyl and morpholinocarbonyl) from mono‐O‐carbamates of 1,3‐dipropyl‐p‐tert‐butylcalix4arene in 65–75% yield. In addition, p‐t‐Bu‐BCC with two N,N‐dimethylcarbamoyl bridge‐substituents was prepared from mono‐O‐carbamate of p‐t‐Bu‐BCC with one N,N‐dimethylcarbamoyl bridge‐substituent by this method in 71% yield. Finally, the racemic p‐t‐Bu‐BCC with morpholinocarbonyl bridge‐substituent was optically resolved into a pair of enantiomers.
AIM: To evaluate the hemostatic effect of topical hemocoagulase spray in digestive endoscopy.METHODS: Eighty-nine patients who developed oozing bleeding during endoscopic treatment from September ...2014 to October 2014 at Center for Digestive Endoscopy, Tianjin Medical University General Hospital were randomly divided into either a study group(n = 39) or a control group(n = 50). The study group was given topical hemocoagulase spray intraoperatively, while the control group was given traditional 8% norepinephrine spray. Hemostatic efficacy was compared between the two groups. Bleeding site, wound cleanliness and perforation were recorded, and the rates of perforation and late bleeding were compared.RESULTS: Successful hemostasis was achieved in 39(100%) patients of the study group and in 47(94.0%) patients of the control group, and there was no significant difference in the rate of successful hemostasis between the two groups. Compared with the control group, after topical hemocoagulase spray in the study group, the surgical field was clearer, the bleeding site was more easily identified, and the wound was cleaner. There was no significant difference in the rate of perforation between the study and control groups(16.7% vs 35.0%, P = 0.477), but the rates oflate bleeding(0% vs 15.8%, P = 0.048) and overall complications(P = 0.032) were significantly lower in the study group.CONCLUSION: Topical hemocoagulase spray has a definite hemostatic effect for oozing bleeding in digestive endoscopy, and this method is convenient, safe, and reliable. It is expected to become a new method for endoscopic hemostasis.
Calcination of Slags for Electroslag Remelting DONG, Yan-wu; JIANG, Zhou-hua; XIAO, Zhi-xin ...
Journal of iron and steel research, international,
08/2010, Letnik:
17, Številka:
8
Journal Article
Recenzirano
The hydrides in industrial lime,alumina,magnesia,and calcium fluoride were investigated through differential thermal analysis and X-ray diffraction,and their mass losses during heating up were ...studied by thermogravimetric analysis method.The results indicate that the industrial alumina,lime,and magnesia,which have more moisture or hydride and mainly include γ-Al2O3,Ca（OH）2,and Mg（OH）2,lose more mass during thermogravimetric analysis process.However,the mass of premelted slag consisting of lime,fluorite,alumina,and magnesia has almost no change,which means no hydride in it.Some relationships for calculating the mass loss were established according to the results of thermogravimetric analysis.These results will be in favor of setting up the rational calcination criterion for slag used in electroslag remelting process.
Abstract The direct C−H to C−N bond conversion at room temperature using heterogeneous visible‐light catalysts is both interesting and challenging. Herein, we disclosed visible‐light‐promoted ...hydroacylation of azodicarboxylates using uranyl‐organic framework UO 2 (OH)(cpt) n ( Ucpt , Hcpt=4‐(4′‐carboxyphenyl)‐1,2,4‐triazole) as an efficient heterogeneous hydrogen atom transfer photocatalyst. In this procedure, diverse benzaldehydes are compatible using dimethyl carbonate (DMC) as a green solvent at room temperature, achieving direct conversion of C−H to C−N bond with excellent yields (up to 99%). The key to excellent catalytic activity lies in the fact that the coordination of the conjugated ligand changes the HOMO energy level of Ucpt , resulting in more active sites and longer‐lived photo excitons. Simultaneously, this heterogeneous photocatalyst can be easily separated and reused for seven successive cycles and maintain high catalytic activity, showing outstanding sustainability and recyclability.
microRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic and abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield and blast disease resistance ...via different modules. Overexpression of miR1432 leads to compromised resistance and decreased yield, whereas blocking miR1432 using a target mimic of miR1432 results in enhanced resistance and yield. miR1432 suppresses the expression of
LOC_Os03g59790
, which encodes an EF-hand family protein 1 (
OsEFH1
). Overexpression of
OsEFH1
leads to enhanced rice resistance but decreased grain yield. Further study revealed that miR1432 and
OsEFH1
are differentially responsive to chitin, a fungus-derived pathogen/microbe-associated molecular pattern (PAMP/MAMP). Consistently, blocking miR1432 or overexpression of
OsEFH1
improves chitin-triggered immunity responses. In contrast, overexpression of
ACOT
, another target gene regulating rice yield traits, has no significant effects on rice blast disease resistance. Altogether, these results indicate that miR1432 balances yield and resistance via different target genes, and blocking miR1432 can simultaneously improve yield and resistance.
The ramified growth of lithium dendrites and their enormous volume change upon cycling bear the primary responsibility for the poor cyclability and serious safety of lithium metal batteries. Herein, ...a low volume change composite lithium metal anode is realized by encaging Li into a hybrid host featuring a 3D conducting scaffold with a metal-organic frameworks (MOFs) coating. The scaffold with high pore volume is favorable to accommodate Li with high areal capacity and alleviate the variation in electrode dimensions. The MOFs layer with abundant interconnected micropores serving as “ion sieve” can boost uniform distribution of Li ions while its high Young's modulus (>32 GPa) can arrest dendrite propagation. The thus-formed anode displays a negligible dimension variation (<5%), enabling stable cycling in both symmetric cells (>1000 h) and full cells (>200 cycle-life). This study may open up new directions to construct safe Li metal batteries through an industry-adoptable technology.
A low volume change composite lithium metal anode with dendrite free is constructed by integrating a 3D conducting scaffold with a metal-organic frameworks (MOFs) coating. Display omitted
•A low volume change composite lithium metal anode is constructed by encaging Li into a hybrid host.•The host, with a dual-layer structure, can alleviate electrode volume variation and suppress dendritic Li growth.•The thus-formed Li anode works stably in both symmetric cells and full cells.
Phyllosphere contains various microorganisms that may harbor diverse antibiotic resistance genes (ARGs). However, we know little about the composition of antibiotic resistome and the factors ...influencing the diversity and abundance of ARGs in the phyllosphere. In this study, 16S rRNA gene amplicon sequencing and high-throughput quantitative PCR approaches were employed to investigate the effects of long-term (over 10 years) organic fertilization on the phyllosphere bacterial communities and antibiotic resistome. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes dominated in the phyllosphere bacterial communities. Long-term application of sewage sludge and chicken manure altered the phyllosphere bacterial community composition, with a remarkable decrease in bacterial alpha-diversity. A total of 124 unique ARGs were detected in the phyllosphere. The application of sewage sludge and chicken manure significantly increased the abundance of ARGs, with a maximum 2638-fold enrichment. Variation partitioning analysis (VPA) together with network analysis indicated that the profile of ARGs is strongly correlated with bacterial community compositions. These results improve the knowledge about the diversity of plant-associated antibiotic resistome and factors influencing the profile of ARGs in the phyllosphere.
Display omitted
•Sewage sludge and manure amendment increased the occurrence and abundance of ARGs in phyllosphere.•Bacterial community change was the major drives in shaping ARGs in the phyllosphere.•Soil may serve as an antibiotic resistome reservoir for phyllosphere.
Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible ...for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRNA-targeting endoribonuclease: SLFN13, a member of the Schlafen family. Structural study reveals a unique pseudo-dimeric U-pillow-shaped architecture of the SLFN13 N'-domain that may clamp base-paired RNAs. SLFN13 is able to digest tRNAs and rRNAs in vitro, and the endonucleolytic cleavage dissevers 11 nucleotides from the 3'-terminus of tRNA at the acceptor stem. The cytoplasmically localised SLFN13 inhibits protein synthesis in 293T cells. Moreover, SLFN13 restricts HIV replication in a nucleolytic activity-dependent manner. According to these observations, we term SLFN13 RNase S13. Our study provides insights into the modulation of translational machinery in high eukaryotes, and sheds light on the functional mechanisms of the Schlafen family.
We performed this review to clarify which dietary and lifestyle factors are related to gastroesophageal reflux disease. Through a systematic search of the PubMed, EMBASE, China National Knowledge ...Infrastructure (CNKI), and Chinese BioMedical Literature (CBM) databases, we identified articles with clear definitions of GERD, including nonerosive gastroesophageal reflux disease (NERD), reflux esophagitis (RE) and Barrett's esophagus (BE), that included dietary and lifestyle factors as independent factors affecting the onset of GERD (expressed as odds ratios (ORs) or relative risks (RRs) and 95% confidence intervals (CIs)). Due to heterogeneity among the studies, we used descriptive statistical analyses to analyze and synthesize each outcome based on the disease type. In total, 72 articles were included, conducted in ten Western countries (26 articles in total) and nine Eastern countries (46 articles in total). We categorized dietary factors into 20 items and lifestyle factors into 11 items. GERD is related to many irregular dietary and lifestyle habits (such as a habit of midnight snacking: OR=5.08, 95% CI 4.03-6.4; skipping breakfast: OR=2.7, 95% CI 2.17-3.35; eating quickly: OR=4.06, 95% CI 3.11-5.29; eating very hot foods: OR=1.81, 95% CI 1.37-2.4; and eating beyond fullness: OR=2.85, 95% CI 2.18-3.73). Vegetarian diets (consumption of nonvegetarian food (no/yes); OR=0.34, 95% CI 0.211-0.545) and no intake of meat (OR=0.841, 95% CI 0.715-0.990) were negatively related to GERD, while meat (daily meat, fish, and egg intake: OR=1.088, 95% CI 1.042-1.135) and fat (high-fat diet: OR=7.568, 95% CI 4.557-8.908) consumption were positively related to GERD. An interval of less than three hours between dinner and bedtime (OR=7.45, 95% CI 3.38-16.4) was positively related to GERD, and proper physical exercise (physical exercise >30 minutes (>3 times/week): OR=0.7, 95% CI 0.6-0.9) was negatively correlated with GERD. Smoking (OR=1.19, 95% CI 1.12-1.264), alcohol consumption (OR=1.278, 95% CI 1.207-1.353) and mental state (poor mental state: OR=1.278, 95% CI 1.207-1.353) were positively correlated with GERD. RE (vitamin C: OR=0.46, 95% CI=0.24-0.90) and BE (vitamin C: OR=0.44,95% CI 0.2-0.98; vitamin E: OR=0.46, 95% CI 0.26-0.83) were generally negatively correlated with antioxidant intake. In conclusion, many dietary and lifestyle factors affect the onset of GERD, and these factors differ among regions and disease types. These findings need to be further confirmed in subsequent studies.
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