In this paper, firstly, the topic structure of the Chinese language and literature is explained, and the micro topic structure representation system is constructed by using the main statement bit ...structure theory and the main bit advancement model. Secondly, the word2vec network is used to extract the features of Chinese language and literature elements, and a multi-feature fusion classification recognition model is constructed based on SVM. Finally, the corpus was used to validate the example. The results show that the percentage of radial primary advancement pattern of micro topic structure is the highest and reaches 94.13%, and the recognition accuracy of SVM-based multi-feature fusion recognition classification model for micro topic structure is improved by 12.04%, 18.29% and 7.45% respectively compared with BTM algorithm, LDA algorithm and K-means algorithm.
The aim of this study is to evaluate the relationship between antinuclear antibody (ANA) titer and specificity, as well as the relationship between the number of positive-autoantibodies (AAbs) in ...antinuclear antibodies (ANAs) and specificity for systemic lupus erythematosus (SLE), so as to explore their significance in the diagnosis of SLE. A total of 1297 patients with ANA results was enrolled in this study, including 148 patients with SLE patients. The sensitivity, specificity, sensitive likelihood ratio and specific likelihood ratio of indicators in SLE were determined by receiver-operator characteristic (ROC) curve after measurement of ANA and ANAs by indirect immunofluorescence (IIF) and immunoblotting, respectively. ROC analysis showed that the specificity of ANA titer ≥ 1 +, ≥ 2 + and ≥ 3 + for SLE was estimated to be 81.29%, 90.69% and 96.52% respectively, with a increased titer-specific likelihood ratio (5.16, 9.29 and 19.60, respectively). The specificity of the number of positive-AAbs ≥ 1, ≥ 2 and ≥ 3 in ANAs for SLE was estimated to be 80.42%, 94.95% and 99.3% respectively, with a increased number-specific likelihood ratio (4.8, 15.26 and 72.48, respectively). The estimated sensitivity of the number of positive-AAbs ≥ 3, AnuA and anti-rRNP was higher than that of anti-Sm (p < 0.01) (50.68%, 41.89% and 31.76% vs. 16.89%, respectively), while there was no significant difference in their specificity (99.3%, 99.74% and 99.56% vs. 99.74%, respectively) (p > 0.05). High titers of ANA and the presence of multiple AAbs in ANAs are highly specific for SLE and highly suggestive of SLE. The likelihood of SLE can be assessed by ANA titer and the number of positive-AAbs in ANAs.
Anaerobic decolorization of azo dyes has been evidenced to be an economical and effective pretreatment method, but its generally limited by the low decolorization efficiency, especially for ...biodecolorization sulfonated azo dyes. In this study, magnetite nanoparticles (MNPs) as a conductive material, was coupled into anaerobic system for enhancing decolorization of sulfonated azo dyes, i.e., methyl orange (MO), with technology feasibility and system stability emphasized. The results showed that the anaerobic decolorization capacity was significantly enhanced with addition of MNPs (at dose of 1 g/L), where the efficiencies of MO decolorization and aromatic amines formation were as high as 97.28 ± 0.78 % and 99.44 ± 0.25%, respectively. In addition, both electron transport system activity and sludge conductivity were also significantly improved, suggesting that a direct extracellular electron transfer had been successfully established via MNPs as RMs. Under continuous-flow experiments, addition of MNPs not only improved anaerobic system resistance environmental stress (e.g., high MO concentration, low hydraulic retention time and low co-substance concentration) but also accelerated sludge granulation. The relative abundance of functional species related to dissimilatory iron reduction and MO biodegradation were also enriched under MNPs stimulation. The observed long-term stable performance suggests the full-scale application potential of this coupled system for treatment of wastewater containing sulfonated azo dyes.
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•>Anaerobic decolorization capacity was enhanced with addition of MNPs.•>A stable and fast EET was established by addition of conductivity MNPs.•>The ability to resist environmental stress was enhanced in the coupled system.•>The sludge granulation process was accelerated under MNPs stimulation.•>Species related to MO biodegradation and ferric ions reduction were enriched.
The occurrence of human infections caused by avian H9N2 influenza viruses has raised concerns regarding the potential for human epidemics and pandemics. The molecular basis of viral adaptation to a ...new host needs to be further studied. Here, the bases of nucleotides 627 and 701 of PB2 were changed according to the uncoverable purine-to-pyrimidine transversion to block the development of PB2 627K and 701N mutations during serial passaging in mice. The purpose of this experiment was to identify key adaptive mutations in polymerase and NP genes that were obscured by the widely known host range determinants PB2 627K and 701N. Mouse-adapted H9N2 variants were obtained via twelve serial lung-to-lung passages. Sequence analysis showed that the mouse-adapted viruses acquired several mutations within the seven gene segments (PB2, PB1, PA, NP, HA, NA, and NS). One variant isolate with the highest polymerase activity possessed three substitutions, PB2 S155N, PA S49Y and D347G, which contributed to the highly virulent and mouse-adaptative phenotype. Further studies demonstrated that these three mutations resulted in increased polymerase activity, viral transcription and replication in mammalian cells, severe interstitial pneumonia, excessive inflammatory cellular infiltration and increased growth rates in mice. Our results suggest that the substitution of these three amino acid mutations may be an alternative strategy for H9N2 avian influenza viruses to adapt to mammalian hosts. The continued surveillance of zoonotic H9N2 influenza viruses should also include these mammalian adaptation markers as part of our pandemic preparedness efforts.
The incidence rate of nasopharyngeal carcinoma (NPC) is the highest among the malignant tumors of otorhinolaryngology, posing a huge burden to public health. Long noncoding RNAs (lncRNAs) exert an ...important role in tumorigenesis and the progression of various cancers. The present study found that HOXC-AS1 was highly expressed in NPC and in NPC cell lines, suggesting a critical role of HOXC-AS1 in NPC progression. In addition, the abundance of HOXC-AS1 was negatively correlated with the prognosis of NPC. To molecularly dissect the mechanism of HOXC-AS1 in NPC progression, we knocked down the expression of HOXC-AS1 in HNE1 and C666-1 cells. Then, we employed CCK8, colony-formation experiment and Transwell to investigate how the cell performed when HOXC-AS1 was knocked down. It could be observed that HOXC-AS1 knockdown decreases cell proliferation, migration and invasion, but induces cell apoptosis in NPC. We found that HOXC-AS1 could sponge miR-4651 subsequently binding FOXO6 and inhibiting its expression. Therefore, HOXC-AS1/miR-4651/FOXO6 may form a competing endogenous RNA (ceRNA) network that promotes NPC progression. In conclusion, our study demonstrates that HOXC-AS1 promotes NPC progression by sponging miR-4651 and regulating FOXO6 expression, thus providing potential pharmaceutical targets for developing new NPC treatments.
Age-related hearing loss (ARHL) is the most common sensory disorder associated with human aging. Chronic inflammation is supposed to be an important contributor to ARHL. Yet, the underlying ...mechanisms of developing cochlear inflammation are still not well understood. In this study, we found that the inflammation, endoplasmic reticulum (ER) stress and necroptosis signalings are activated in the cochlea of aged C57BL/6 mice. ER stress activator tunicamycin (TM) induced necroptosis in cochlear HEI-OC1 cells and cochlear explants, while necroptosis inhibitors protected cochlear cells from ER stress-induced cell death. The antioxidants inhibited necroptosis and protected HEI-OC1 cells from TM insults. Necroptotic HEI-OC1 cells promoted the activation of the co-cultured macrophages via Myd88 signaling. Moreover, necroptosis inhibitor protected from TM-induced hearing loss, and inhibited inflammation in C57BL/6 mice. These findings suggest that ER stress-induced necroptosis promotes cochlear inflammation and hearing loss. Targeting necroptosis serves as a potential approach for the treatment of cochlear inflammation and ARHL.
•ER stress, necroptosis and inflammation signalings are activated during cochlear aging.•ER stress induces cochlear cell necroptosis partially by ROS production.•Necroptotic cochlear cells activate macrophages via Myd88 signaling.•In mouse, ER stress-induced necroptosis promotes cochlear inflammation and hearing loss.
Cobalamin deficiency is believed to be related to disturbances in cell division, neuropathy, nervous system disease and pernicious anemia. Elderly people are usually advised to supplement their diets ...with cobalamin. As cobalamin has several forms, the effects of different forms of cobalamin on gut microbiota were investigated in this study. After 7 days of supplementation, methylcobalamin had reduced the diversity of gut microbiota compared to that in the control and cyanocobalamin groups. After supplementation with methylcobalamin, the percentage of
spp. had increased to 45.54%, while the percentages of
spp.,
spp. and
spp. had declined to 11.15, 9.34, and 2.69%, respectively. However, cyanocobalamin had different influences on these bacteria. Both cobalamins increased the amount of short-chain fatty acids, particularly butyrate and propionic acid. The cyanocobalamin group showed increased activity of cellulase compared with that in the other two groups. According to CCA and PICRUSt analysis, methylcobalamin had a positive correlation with
bacteria, propionic acid, and butyrate. Methylcobalamin promoted lipid, terpenoid, and polyketide metabolism by gut bacteria, promoted the degradation of exogenous substances, and inhibited the synthesis of transcription factors and secondary metabolites. Our results indicate that the various forms of cobalamin in the food industry should be monitored and regulated, because the two types of cobalamin had different effects on the gut microbiome and on microbial metabolism, although they have equal bio-activity in humans. Given the effects of methylcobalamin on gut microbiota and microbial metabolism, methylcobalamin supplementation should be suggested as the first option.
The sox2 expressing (sox2
) progenitors in adult mammalian inner ear lose the capacity to regenerate while progenitors in the zebrafish lateral line are able to proliferate and regenerate damaged HCs ...throughout lifetime. To mimic the HC damage in mammals, we have established a zebrafish severe injury model to eliminate both progenitors and HCs. The
expressing (
) HC precursors were the main population that survived post severe injury, and gained sox2 expression to initiate progenitor regeneration. In response to severe injury,
was activated to upregulate
transcription. Severe-injury-induced progenitor regeneration was disabled in
or
mutants. In contrary, overexpression of
initiated the recovery of sox2
progenitors. Mechanistically, microRNA
acted downstream of
to activate Wnt pathway for promoting regeneration. Our findings that lin28a is necessary and sufficient to regenerate the exhausted sox2
progenitors shed light on restoration of progenitors to initiate HC regeneration in mammals.
Abstract The molecular mechanisms underlying age-related hearing loss are not fully understood, and currently, there is no treatment for this disorder. MicroRNAs have recently been reported to be ...increasingly associated with age-related diseases and are emerging as promising therapeutic targets. In this study, miR-34a/Sirtuin 1 (SIRT1)/p53 signaling was examined in cochlear hair cells during aging. MiR-34a, p53 acetylation, and apoptosis increased in the cochlea of C57BL/6 mice with aging, whereas an age-related decrease in SIRT1 was observed. In the inner ear HEI-OC1 cell line, miR-34a overexpression inhibited SIRT1, leading to an increase in p53 acetylation and apoptosis. Moreover, miR-34a knockdown increased SIRT1 expression and diminished p53 acetylation, and apoptosis. Additionally, resveratrol, an activator of SIRT1, significantly rescued miR-34a overexpression-induced HEI-OC1 cell death and significantly reduced hearing threshold shifts and hair cell loss in C57BL/6 mice after a 2-month administration. Our results support a link between age-related cochlear hair cell apoptosis and miR-34a/SIRT1/p53 signaling, which may serve as a potential target for age-related hearing loss treatment.
•Online property brokerage platform entry reduces second-hand housing price.•The impact is weakened with the increase in new house price.•The impact is weakened with the increase in total ...households.•Platform entry may boost the sales of second-hand houses.
Despite the popular emergence of online property brokerage platforms in the second-hand housing market, little is known about whether and how these platforms would influence the market, especially in terms of housing price. We thus assemble and analyze data from multiple sources about the entry of a representative Chinese platform (Lianjia.com) and local housing market for empirical validation. Our analysis found that platform entry may decrease the price of second-hand houses, and the impact would be weakened with the increase in new house price and total households. Moreover, platform entry eventually may boost the sales of second-hand houses. These notable findings offer several important contributions. First, our research is one of the first to explore the intricate impact of online property brokerage platforms on housing price. Second, our research is one of the few to study the impact of online intermediaries on the sales price of large consumer goods. Third, we further uncover some contingent factors and subsequent downstream outcome of this impact. Practically, our findings also offer important implications to practitioners and policy makers.
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