Gelatinous transformation of the bone marrow in a 79-year-old patient was observed. It was associated with acute leukemia diagnosed on the biopsy of a vertebral body, indicated by previous ...Indium-transferrin scintigraphy. Though the lesion is usually associated with severe weight loss and cachexia, the rare cases of gelatinous transformation reported among patients with malignant disease are summarized. It is suggested that metastatic changes by blast cells in acute leukemia may lead to gelatinous transformation of bone marrow but this remains to be investigated.
Comme pour tout examen biologique, la qualité des analyses parasitologiques et mycologiques implique la maîtrise de la phase pré-analytique qui comporte la réalisation du prélèvement, son ...conditionnement et son transport. En laboratoires d'analyses médicales privés ou hospitaliers, la phase pré-analytique implique différents intervenants et doit répondre à des obligations biologiques de conditions de prélèvement, d'acheminement et de conservation, ensemble de mesures qui sont toutes désormais codifiées dans le Guide de bonne exécution des analyses (GBEA). Ce travail propose un plan de procédure et des tableaux récapitulatifs sur la méthodologie des prélèvements réservés aux biologistes et sur les recherches, pouvant être effectuées sur chaque type d'échantillon ainsi que le conditionnement du prélèvement, le délai d'acheminement au laboratoire et sa conservation en cas d'analyse différée.
As any biological analysis, the quality of parasitological and mycological analysis requires the control of the preanalytic phase which comprises execution, conditioning and transport to laboratory of the sample. In private or hospital analytical laboratories, the preanalytic phase implies different professionals and must meet biological requirements for the conditions of sampling, forwarding and conservation. This is a series of measures which are henceforth codified in the Guide de bonne exécution des analyses
(GBEA). This work proposes a plan of procedure and charts on the methodology of sampling for biologists and on the research that could be carried out on each type of sample as well as the conditioning, forwarding time to the laboratory and conservation in the event of a delayed analysis.
Malaria: what about exposure prophylaxis? Lundwall, Eric; Pennetier, Cédric; Corbel, Vincent ...
La revue du praticien,
2005-Apr-30, Letnik:
55, Številka:
8
Magazine Article
Malaria would not exist without mosquito bites. The growing resistance to antimalarial drugs has led to an increasing role of personal protection measures. However, insect repellents are not ...evaluated as medicinal products, and many products available on the market contain active substances that have been poorly evaluated or present in insufficient doses. The efficacy of the most active insect repellents, DEET and icaridin/KBR 3023, like that of medicinal products, is limited by the subject's compliance, bearing in mind that the Anopheles bite is painless and that humans are primarily bothered by the nuisance factor of the mosquito.
Environmental DNA (eDNA) metabarcoding is increasingly used to study the present and past biodiversity. eDNA analyses often rely on amplification of very small quantities or degraded DNA. To avoid ...missing detection of taxa that are actually present (false negatives), multiple extractions and amplifications of the same samples are often performed. However, the level of replication needed for reliable estimates of the presence/absence patterns remains an unaddressed topic. Furthermore, degraded DNA and PCR/sequencing errors might produce false positives. We used simulations and empirical data to evaluate the level of replication required for accurate detection of targeted taxa in different contexts and to assess the performance of methods used to reduce the risk of false detections. Furthermore, we evaluated whether statistical approaches developed to estimate occupancy in the presence of observational errors can successfully estimate true prevalence, detection probability and false‐positive rates. Replications reduced the rate of false negatives; the optimal level of replication was strongly dependent on the detection probability of taxa. Occupancy models successfully estimated true prevalence, detection probability and false‐positive rates, but their performance increased with the number of replicates. At least eight PCR replicates should be performed if detection probability is not high, such as in ancient DNA studies. Multiple DNA extractions from the same sample yielded consistent results; in some cases, collecting multiple samples from the same locality allowed detecting more species. The optimal level of replication for accurate species detection strongly varies among studies and could be explicitly estimated to improve the reliability of results.
Here we present the results from a bourgeoning tool, the analyses of lake sediment DNA applied to the reconstruction of agriculture and landscape histories (plant and mammal DNA) in the French Alps. ...As the tool is pioneer, the first part of the manuscript is focused on the study of the conditions to archive the DNA in lake sediments and on the reliability of the data. Results suggest that lakes submitted to high detrital inputs are more favourable to the record of DNA coming from the catchment. Moreover, we show that DNA and pollen analyses are complementary. Combining these two approaches, we can distinguish the local from the more regional landscape evolution. The reconstruction of local landscapes and the access to the composition of livestock farming are the main advantages of the lake sediment DNA tool. With this innovative tool, we can reconstruct a detailed picture of the alpine agriculture dynamic in space and time, as well as study their effects on the environment.
L’article proposé ici présente les résultats d’un outil en plein essor, l’ADN sédimentaire lacustre appliqué à la reconstitution de l’histoire des activités agricoles et des paysages (ADN des plantes et des mammifères) dans les Alpes françaises. étant donné le caractère novateur de l’outil, une partie de l’article est aussi consacrée à une réflexion sur les modalités d’archivage de l’ADN dans les sédiments et sur la fiabilité des données. Les résultats suggèrent que les lacs soumis à des apports détritiques importants sont plus favorables à l’enregistrement de l’ADN provenant du bassin versant. Nous montrons également que les analyses ADN et palynologiques sont complémentaires. Combinées, elles permettent de mieux discerner l’évolution du paysage local de celle du paysage plus régional. L’aspect local de la reconstitution des paysages à l’aide de l’ADN et la détermination de la composition des animaux d’élevage constituent les principales plus-values de l’approche ADN. Ceci nous permet d’avoir non seulement une image plus détaillée de la dynamique des activités agricoles développées par les populations alpines, mais aussi de leurs effets sur l’environnement.
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