Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland ...Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.
Mapping wetlands with high spatial and thematic accuracy is crucial for the management and monitoring of these important ecosystems. Wetland maps in New Brunswick (NB) have traditionally been ...produced by the visual interpretation of aerial photographs. In this study, we used an alternative method to produce a wetland map for southern New Brunswick, Canada, by classifying a combination of Landsat 8 OLI, ALOS-1 PALSAR, Sentinel-1, and LiDAR-derived topographic metrics with the Random Forests (RF) classifier. The images were acquired in three seasons (spring, summer, and fall) with different water levels and during leaf-off/on periods. The resulting map has eleven wetland classes (open bog, shrub bog, treed bog, open fen, shrub fen, freshwater marsh, coastal marsh, shrub marsh, shrub wetland, forested wetland, and aquatic bed) plus various non-wetland classes. We achieved an overall accuracy classification of 97.67%. We compared 951 in-situ validation sites to the classified image and both the 2106 and 2019 reference maps available through Service New Brunswick. Both reference maps were produced by photo-interpretation of RGB-NIR digital aerial photographs, but the 2019 NB reference also included information from LiDAR-derived surface and ecological metrics. Of these 951 sites, 94.95% were correctly identified on the classified image, while only 63.30% and 80.02% of these sites were correctly identified on the 2016 and 2019 NB reference maps, respectively. If only the 489 wetland validation sites were considered, 96.93% of the sites were correctly identified as a wetland on the classified image, while only 58.69% and 62.17% of the sites were correctly identified as a wetland on the 2016 and 2019 NB reference maps, respectively.
Recent developments in biocatalysis, where implementation beyond the laboratory has been demonstrated, are explored: the use of transglutaminases to modify foods, reduce allergenicity and produce ...advanced materials, lipases for biodiesel production, and transaminases for biochemical production. The availability and application of enzymes at pilot and larger scale opens up possibilities for further improvements of biocatalyst-based processes and the development of new processes. Enzyme production, stability, activity, re-use, and product retrieval are common challenges for biocatalytic processes. We explore recent advances in biocatalysis within the process chain, such as protein engineering, enzyme expression, and biocatalyst immobilization, in the context of these challenges.
This paper gives an overview of development of the EU-bioeconomy, 2014-2020. The Vision of the new Circular Bio-based Economy, CBE is presented: Unlocking the full potential of all types of ...sustainably sourced biomass, crop residues, industrial side-streams, and wastes by transforming it into value-added products. The resulting product portfolio consists of a wide spectrum of value-added products, addressing societal and consumer needs. Food and feed, bio-based chemicals, materials, health-promoting products; and bio-based fuels. The pillars of CBE are described, including biotechnology, microbial production, enzyme technology, green chemistry, integrated physical/chemical processing, policies, conducive framework conditions and public private partnerships. Drivers of CBE are analyzed: Biomass supply, biorefineries, value chain clusters, rural development, farmers, foresters and mariners; urgent need for climate change mitigation and adaptation, and stopping biodiversity loss. Improved framework conditions can be drivers but also obstacles if not updated to the era of circularity. Key figures, across the entire BBI-JU project portfolio (2014-2020) are provided, including expansion into biomass feedstocks, terrestrial and aquatic, and an impressive broadening of bio-based product portfolio, including higher-value, health-promoting products for man, animal, plants and soil. Parallel to this, diversification of industrial segments and types of funding instruments developed, reflecting industrial needs and academic research involvement. Impact assessment is highlighted. A number of specific recommendations are given; e.g., including international win/win CBE-collaborations, as e.g., expanding African EU collaboration into CBE. In contrast to fossil resources biological resources are found worldwide. In its outset, circular bio-based economy, can be implemented all over, in a just manner, not the least stimulating rural development.
Polyether block amides (PEBA) composite membranes were prepared by dispersing different two dimensional (2D) layered nanomaterials such as graphene, graphene oxide and molybdenum disulphide (MoS2) in ...PEBA matrix. These composite membranes were applied for the pervaporation separation of butyric acid produced by anaerobic digestion (AD) of perennial grass. Among all the tested membranes PEBA–graphene membrane showed the best performance with butyric acid flux og 24.3g/m2h and separation factor of 21. Further on varying the graphene content in the membranes showed improved separation efficiency with increased thermal and mechanical properties of the membranes.
A standard blood prime for cardiopulmonary bypass (CPB) in congenital cardiac surgery may possess non-physiologic values for electrolytes, glucose, and lactate. Pre-bypass Ultrafiltration (PBUF) can ...make these values more physiologic and standardized prior to bypass initiation. We aimed to determine if using PBUF on blood primes including packed red blood cells and thawed plasma would make prime values more predictable and physiologic. Additionally, we aimed to evaluate whether the addition of PBUF had an impact on outcome measures.
Retrospective review of consecutive patients ≤ 1 year of age undergoing an index cardiac operation on CPB between 8/2017 and 9/2021. As PBUF was performed at the perfusionists' discretion, a natural grouping of patients that received PBUF vs. those that did not occur. Differences in electrolytes, glucose, and lactate were compared at specific time points using Fisher's exact test for categorical variables and the Wilcoxon rank sum test for continuous variables. Clinical outcomes were also assessed.
In both cohorts, the median age at surgery was 3 months and 47% of patients were female; 308/704 (44%) of the PBUF group and 163/414 (39%) of the standard prime group had at least one preoperative risk factor. The proportion of PBUF circuits which demonstrated more physiologic values for glucose (318 45%), sodium (434, 62%), potassium (688 98%), lactate (612 87%) and osmolality (595 92%) was significantly higher when compared to standard prime circuit levels for glucose (8 2%), sodium (13 3%, potassium (150 36%), lactate (56 13%) and osmolality (23 6%) prior to CPB initiation. There were no differences in clinical outcomes or rates of major adverse events between the two cohorts.
PBUF creates standardized and more physiologic values for electrolytes, glucose, and lactate before the initiation of bypass without significant impacts on in-hospital outcomes.
Summary
In the present work, poly (3‐hydroxybutyrate) (PHB) blends were prepared by melt blending with biobased oligomeric diisocyanate synthesised from hexamethelene diisocyanate (Tolonate XFLO100 ...(TXF100)). PHBTXF100 blends with four different weight fraction (10, 15, 20 and 25 phr) of TXF100 were prepared and characterized for mechanical, thermal and thermomechanical properties. Strain at break and tensile toughness of PHBTXF10025 blend were improved by 200% and 193%, respectively. PHBTXF100 blends showed a broad glass transition temperature with a decreased onset temperature compared to PHB. PHBTXF100 blends exhibited low crystallinity than PHB, and was further decreased with an increased weight fraction of TXF100. Elastic and storage moduli PHBTXF100 blends were decreased compared to PHB, and correlated to their low degree of crystallinity. The changes in mechanical properties of PHBTXF100 blends were mainly due to efficient weakening of intermolecular interactions in the amorphous regions of PHB, and also a slight decrease in crystalline order of PHB.
A 1-year old male patient with Williams syndrome and multiple prior interventions presented for surgical repair of his descending aorta (DA) through a left thoracotomy. Concerns for significant ...bleeding and spinal cord protection led the care team to consider a left heart bypass (LHB) circuit with options for pump sucker use, heat exchange capacity, and the possibility of converting to traditional cardiopulmonary bypass (CPB). A traditional CPB circuit with a roller-head arterial pump was assembled with a bypass line around the cardiotomy venous reservoir (CVR). Excluding the CVR with this line allowed for a closed LHB circuit. A second pump head was integrated to both recirculate the CVR volume and to serve as a means for controlled volume administration to the closed LHB circuit. Pump sucker return directed to the CVR could easily be transfused back to the patient. The patient was placed on the hybrid LHB circuit and cooled to 32°C. DA clamps were placed. Upper body dynamic blood pressure was managed for a target mean of 50 mmHg, the left atrial pressure (LAP) was maintained in the 5-7 mmHg range, and the nonpulsatile lower body blood pressure was targeted at 40-50 mmHg. Cerebral near-infrared spectroscopy (NIRS) helped guide volume and pressure management. The surgeons placed two long-segment patches on the DA, moving clamps as needed. The patient was rewarmed and separated from the hybrid LHB circuit after 82 minutes. Closed circuit LHB can be provided with a roller-head hybrid circuit incorporating an oxygenator for gas exchange, central cooling and warming, and arterial line filtration along with a CVR for pump sucker use and controlled transfusion to the patient.
A 1-year old male patient with Williams syndrome and multiple prior interventions presented for surgical repair of his descending aorta (DA) through a left thoracotomy. Concerns for significant ...bleeding and spinal cord protection led the care team to consider a left heart bypass (LHB) circuit with options for pump sucker use, heat exchange capacity, and the possibility of converting to traditional cardiopulmonary bypass (CPB). A traditional CPB circuit with a roller-head arterial pump was assembled with a bypass line around the cardiotomy venous reservoir (CVR). Excluding the CVR with this line allowed for a closed LHB circuit. A second pump head was integrated to both recirculate the CVR volume and to serve as a means for controlled volume administration to the closed LHB circuit. Pump sucker return directed to the CVR could easily be transfused back to the patient. The patient was placed on the hybrid LHB circuit and cooled to 32°C. DA clamps were placed. Upper body dynamic blood pressure was managed for a target mean of 50 mmHg, the left atrial pressure (LAP) was maintained in the 5–7 mmHg range, and the nonpulsatile lower body blood pressure was targeted at 40–50 mmHg. Cerebral near-infrared spectroscopy (NIRS) helped guide volume and pressure management. The surgeons placed two long-segment patches on the DA, moving clamps as needed. The patient was rewarmed and separated from the hybrid LHB circuit after 82 minutes. Closed circuit LHB can be provided with a roller-head hybrid circuit incorporating an oxygenator for gas exchange, central cooling and warming, and arterial line filtration along with a CVR for pump sucker use and controlled transfusion to the patient.
Wetland management by the Eastern Habitat Joint Venture (EHJV) has focused primarily on water level control to increase the amount of available brood-rearing habitat for waterfowl along the Saint ...John River floodplain in New Brunswick. Impounded wetlands make up approximately 13% of the Saint John River Floodplain complex. Study objectives included an evaluation of waterfowl brood, and wetland obligate bird use of impoundments and seasonally flooded wetlands within the Saint John River floodplain. Historical water level data and a GIS wetlands inventory were used to estimate the duration of flooding on seasonally flooded wetland habitats, and the distribution and relative amount of brood-rearing habitat throughout the breeding period by region. Aerial brood surveys and call response surveys were used to estimate the relative abundance of waterfowl broods and breeding wetland obligate birds respectively. The overall density of waterfowl broods was greater on impoundments than on seasonally flooded wetlands during both years of study but varied by site. Mean species richness of wetland obligate birds was significantly greater on impoundments than on seasonally flooded wetland habitat. Generally, use of seasonally flooded wetlands by wetland obligate birds during late summer declined while the use of impoundments increased. Current habitat management for waterfowl appears to be compatible with habitat requirements of wetland obligate birds by increasing the availability of interspersed open water and emergent vegetation throughout the breeding season. A watershed-based analysis of wetland habitat suggests future wetland management should focus on enhancing current impoundments within the Saint John River floodplain. Resources must be secured for maintenance and water level manipulation within existing managed wetlands rather than the construction of additional impoundments. Further evaluation of the distribution of wetland habitat types in the province is essential to identifying focus areas for waterbird conservation throughout NB.
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