In addition to their pigment properties, the potential health benefits of anthocyanins have made them a subject of interest in recent years. This study aimed to obtain purified anthocyanin fractions ...from native Mexican black bean cultivars using Amberlite XAD-7 resin column and HPCCC and evaluate their anti-inflammatory properties using RAW 264.7 cells. The major anthocyanins in the purified anthocyanin fractions were delphinidin 3-glucoside (61.8%), petunidin 3-glucoside (25.2%), and malvidin 3-glucoside (12.2%). Purified anthocyanin fractions at 12.5 μg/mL effectively prevented LPS-induced ERK1/ERK2 phosphorylation and reduced the protein expression of COX-2 and mRNA expression of iNOS. Results showed that purified anthocyanin fractions have the potential to modulate the inflammatory response by inhibiting the production of pro-inflammatory mediators through the ERK1/ERK2 and NF-κB pathways. This study suggests that anthocyanins from black beans could be used as a natural strategy to help modulate inflammation-associated diseases.
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•Native bean anthocyanins could be used as a strategy to control inflammation.•Purified anthocyanin fractions from black beans were effectively obtained by HPCCC.•Black bean anthocyanins modulate inflammation through the ERK and NF-κB pathways.
Three-dimensional (3D) printing technology is innovatively used in creating customized healthy food for different population groups. This study provided two appropriate food-ink systems with common ...bean protein extract (CBPE) for the syringe-based 3D food printer (0.5 g of sodium alginate, 6 g of gelatin, and 40 g of CBPE in 100 mL of water) and the gear-based 3D food printer (3.5 g of agar, 0.05 g of xanthan, and 12 g of CBPE in 100 mL of water), respectively. Superfine grinding significantly (p < 0.05) decreased the particle size of CBPE and resulted in a reduction in the printability mainly through increasing (p < 0.05) the adhesiveness and the swell powder of food-ink systems, respectively for the syringe-based 3D food printer and the gear-based 3D food printer. The decrease in the stability of printed products by the syringe-based 3D food printer was mainly due to the reduced water binding capacity (p < 0.05) by superfine grinding. Besides, the syringe-based 3D food printer was more suitable for printing CBPE based foods due to its weaker effects on the α-AI activity (p < 0.05). These findings were expected to provide new ideas for the potential application of white common bean protein in 3D food printing technology.
•Two food-ink systems with common bean protein extracts (CBPE) were established.•Superfine grinding of CBPE affected the 3D printability of the food-ink systems.•Superfine grinding of CBPE modified the physical properties of the food-ink systems.•CBPE kept the a-AI activity after 3D printing by the syringe-based printer.
Introduction Genotyping large-scale gene bank collections requires an appropriate sampling strategy to represent the diversity within and between accessions. Methods A panel of 44 common bean ( ...Phaseolus vulgaris L.) landraces from the Alliance Bioversity and The Alliance of Bioversity International and the International Center for Tropical Agriculture (CIAT) gene bank was genotyped with DArTseq using three sampling strategies: a single plant per accession, 25 individual plants per accession jointly analyzed after genotyping ( in silico–pool ), and by pooling tissue from 25 individual plants per accession ( seq-pool ). Sampling strategies were compared to assess the technical aspects of the samples, the marker information content, and the genetic composition of the panel. Results The seq-pool strategy resulted in more consistent DNA libraries for quality and call rate, although with fewer polymorphic markers (6,142 single-nucleotide polymorphisms) than the in silico–pool (14,074) or the single plant sets (6,555). Estimates of allele frequencies by seq-pool and in silico–pool genotyping were consistent, but the results suggest that the difference between pools depends on population heterogeneity. Principal coordinate analysis, hierarchical clustering, and the estimation of admixture coefficients derived from a single plant, in silico – pool , and seq-pool successfully identified the well-known structure of Andean and Mesoamerican gene pools of P. vulgaris across all datasets. Conclusion In conclusion, seq-pool proved to be a viable approach for characterizing common bean germplasm compared to genotyping individual plants separately by balancing genotyping effort and costs. This study provides insights and serves as a valuable guide for gene bank researchers embarking on genotyping initiatives to characterize their collections. It aids curators in effectively managing the collections and facilitates marker-trait association studies, enabling the identification of candidate markers for key traits.
This study showed the significantly differences of basic nutrients and metabolite compounds in nine types of beans involved in soybean, mung bean, pea, and common beans. The metabolomics results ...showed that serval metabolites such as histidine, proline, 3-alanine, and myricetin which could be used to identify different beans. The random forest model showed that amino acid and fatty acid could be used as special indexes to distinguish different types of beans in practice. The different expressed metabolites among different types of beans were involved in various pathways including alanine, aspartate and glutamate metabolism, arginine and proline metabolism, and purine metabolism. The antioxidant activity was significantly different among different types of beans, and the contents of amino acid, coumarin, and polyphenol contributed the antioxidant activities of beans. Together, these results will provide a comprehensive understanding of metabolites in different types of beans and theoretical guideline for the future application of beans.
•The basic nutrient and metabolite compositions were significantly different among different types of beans.•Several metabolites could be used to identify the different types of beans.•Amino acid and fatty acid contents play critical roles in the antioxidant activity in beans.
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•Common bean milk and yogurt were developed, and their bioactivities were assessed.•Phenolics and peptides were characterized in bean products as bioactive compounds.•Yogurts ...contained more antioxidant and anti-inflammatory compounds than milks.•Two γ-glutamyl dipeptides were first reported of their bioactive activities.
Common bean milks and their corresponding probiotic yogurts were developed and subjected to simulated gastrointestinal digestion before characterization and assessment of bioactivities. Only digestates fractions of molecular weight <10 kDa showed strong cellular antioxidant activity and anti-inflammatory activity as demonstrated in suppressed TNF-α-induced IL-8 secretion in Caco-2 and HT-29 cell lines. Phenolic and peptide fractions were purified from the digestates. Yogurts showed significantly stronger cellular antioxidant and anti-inflammatory activities compared to their corresponding milks because of the significantly higher total and individual phenolic and peptide contents (P < 0.05). Eleven phenolic compounds and five amino acids/peptides were identified in the phenolic and peptide fractions, respectively using LC-MS/MS. Among them, two γ-glutamyl-dipeptides are reported for the first time for their antioxidant and anti-inflammatory activities. Peptide fractions of yogurts containing higher concentrations of γ-glutamyl-peptides showed stronger anti-inflammatory activity in Caco-2 cells than in HT-29 cells, suggesting the involvement of a peptide transporter PepT1.
Common bean is a legume important in Mediterranean diet with interesting nutritional food characteristics due to its proteins, fibers and antioxidant activity. There is an increased interest among ...consumers in foods rich in natural antioxidants for maintaining and improving health. Beyond this, beans are a low-price food and an excellent complement for the population with low income. There are some differences in the phenolic and flavonoid compounds content and antioxidant capacity between accessions of common bean. In this study, 15 traditional Portuguese and 1 Greek accessions of Phaseolus vulgaris L. were analyzed. Total phenols, ortho-diphenols and flavonoid contents were investigated and antioxidant activity were measured by different assays (DPPH, ABTS) which may give a better characterization of this activity.
Legumes are the third largest family of angiosperms and the second most important crop class. Legume genomes have been shaped by extensive large-scale gene duplications, including an approximately 58 ...million year old whole genome duplication shared by most crop legumes.
We report the genome and the transcription atlas of coding and non-coding genes of a Mesoamerican genotype of common bean (Phaseolus vulgaris L., BAT93). Using a comprehensive phylogenomics analysis, we assessed the past and recent evolution of common bean, and traced the diversification of patterns of gene expression following duplication. We find that successive rounds of gene duplications in legumes have shaped tissue and developmental expression, leading to increased levels of specialization in larger gene families. We also find that many long non-coding RNAs are preferentially expressed in germ-line-related tissues (pods and seeds), suggesting that they play a significant role in fruit development. Our results also suggest that most bean-specific gene family expansions, including resistance gene clusters, predate the split of the Mesoamerican and Andean gene pools.
The genome and transcriptome data herein generated for a Mesoamerican genotype represent a counterpart to the genomic resources already available for the Andean gene pool. Altogether, this information will allow the genetic dissection of the characters involved in the domestication and adaptation of the crop, and their further implementation in breeding strategies for this important crop.
Uganda's lactating mothers are vulnerable to deficiencies of vitamin A and iron because they consume plant‐based conventional foods such as white‐fleshed sweet potato (WFSP) and non‐iron biofortified ...common bean (NIBCB) that are low in provitamin A (PVA) and iron, respectively. A PVA carotenoid–iron‐rich dish was prepared from a combination of orange‐fleshed sweet potato (OFSP) and iron‐biofortified common bean (IBCB). This study evaluated the perceptions and sensory acceptability of OFSP+IBCB (test food) against WFSP+NIBCB (control food) among lactating mothers in rural Uganda. A total of 94 lactating mothers participated in the study. The sensory attributes (taste, color, aroma, texture, and general acceptability) of test and control foods were rated using a five‐point facial hedonic scale (1 = dislike very much, 2 = dislike, 3 = neutral, 4 = like 5 = like very much). An attribute was acceptable if the participant scored from like to like very much. Focus group discussions (FGDs) were conducted to assess participant perceptions about their future consumption of OFSP+IBCB. The chi‐square test was used to detect the proportion difference for each sensory attribute between OFSP+IBCB and WFSP+NIBCB, while FGD data were analyzed by thematic analysis. Taste, color, and aroma were acceptable to the mothers and not significantly different between OFSP+IBCB and WFSP+NIBCB (p > .05). Participants had positive perceptions of the taste, aroma, and color of the OFSP+IBCB and negative perceptions about the soft texture of OFSP. The lactating mothers had positive perceptions of consuming OFSP+IBCB provided they were accessible, affordable, and feasible to prepare.
A provitamin A carotenoid–iron‐rich composite dish prepared from iron‐biofortified common bean and orange‐fleshed sweet potato is acceptable among lactating mothers in rural Uganda. A figure that best represents the scope of the paper. The image supplied should fit within the dimensions of 50 mm x 60 mm, and be fully legible at this size.
Genetic improvement of drought resistance is one of the main breeding goals for common bean; therefore, molecular markers need to be identified to facilitate drought resistance breeding. In this ...study, we evaluated the proline, trehalose, raffinose and stachyose contents of 210 common bean accessions under two watering conditions and found high variation. The coefficient of variation ranged from 21.21% for proline content to 78.69% for stachyose content under well-watered conditions and from 20.11% for proline content to 50.08% for trehalose content under drought stress. According to our genome-wide association analysis, 32 quantitative trait loci were associated with drought resistance, and seven overlapped with known loci. Four hotspot regions were identified at Pv01, Pv07 and Pv11. A set of candidate genes was identified, including genes encoding MYB, bZIP, bHLH, ERF and protein kinases. Among these genes, Phvul.001G189400, Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP, ERF and WRKY, respectively. These genes have been reported to be involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean. Significant SNPs in six candidate gene regions formed different haplotypes, and phenotypic analysis revealed significant differences among the haplotypes. These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.
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