Background: Turmeric is an age old herbaceous plant belonging to the family Zingiberaceae growing in Tropical Africa and India with huge national and international demands. Conventionally, turmeric ...accessions are characterized using morphological and agronomical traits but less work is done in molecular characterization which is needed for thorough trait identification.
Methods: The investigation was carried out during Kharif 2016-17 and comprised of thirty diverse genotypes of turmeric analyzed for genetic variability and molecular diversity which were evaluated in randomized block design with two replications. Fresh tender leaves were used to isolate DNA and PCR was performed with 9 SSR markers. Result: The values of phenotypic coefficient of variation were slightly higher than genotypic coefficient of variation. Highest heritability was observed for days to maturity, genetic advance was observed highest for green rhizome yield. Out of 9 SSR markers, primer pair 5, 6, 7, 8 and 9 were reported to exhibit 100% polymorphism, whereas; in terms of PIC, primer pairs 6 and 9 were found to be highly efficient ones. The similarity coefficient ranged from 0.44 to 1.00 and dendogram categorized the 30 genotypes into two main clusters.
Coffee is an established plant for its flavor and has high commercial use. In Nepal, the popularity of coffee is increasing for its high economic value. However, its diversity and the status of its ...genetic mapping have not been studied in Nepal. In the present study, the genetic diversity of 28 coffee accessions was assessed by using twenty-four SSR markers with the aim of studying the variation of coffee in accord with the genetic markers from a molecular approach. With the use of DNA extraction and marker selection for its amplification using PCR tools, a total of 81 loci from SSR were identified. Of all SSR 63.22% showed for mean polymorphism. The mean polymorphic information content of SSR was 0.38, which showed low genetic diversity of SSR markers among Coffea genotypes. On the basis of the SSR marker, the unweighted pair group method with arithmetic mean (UPGMA) dendrogram constructed showed a similar group of distribution among 28 accessions, which was further supported by a principle coordinate analysis scatter plot. The phylogenetic relationships among the accessions were assessed by SSR marker, which also showed low diversity in coffee genotypes. Our study demonstrated the use of SSR markers in diversity analysis as the data were informative and highly reproducible for evaluating relationships among coffee cultivars in Nepal. The use of more markers systems and a high genotype pool would have been beneficial in accessing more accurately. Regardless, the information from the phylogenetic relationship study could be useful for breeding, varietal improvement, and for conservation programs.
This paper presents a hand gesture based control of an omnidirectional wheelchair using inertial measurement unit (IMU) and myoelectric units as wearable sensors. Seven common gestures are recognized ...and classified using shape based feature extraction and Dendogram Support Vector Machine (DSVM) classifier. The dynamic gestures are mapped to the omnidirectional motion commands to navigate the wheelchair. A single IMU is used to measure the wrist tilt angle and acceleration in three axis. EMG signals are extracted from two forearm muscles namely Extensor Carpi Radialis and Flexor Carpi Radialis and processed to provide Root Mean Square (RMS) signal. Initiation and termination of dynamic activities are based on autonomous identification of static to dynamic or dynamic to static transition by setting static thresholds on processed IMU and myoelectric sensor data. Classification involves recognizing the activity pattern based on periodic shape of trajectories of the triaxial wrist tilt angle and EMG-RMS from the two selected muscles. Second order Polynomial coefficients extracted from the sensor trajectory templates during specific dynamic activity cycles are used as features to classify dynamic activities. Classification algorithm and real time navigation of the wheelchair using the proposed algorithm has been tested by five healthy subjects. Classification accuracy of 94% was achieved by DSVM classifier on ‘k’ fold cross validation data of 5 users. Classification accuracy while operating the wheelchair was 90.5%.
Masa pandemi Covid-19 yang menyebabkan kondisi resesi global dunia berimbas pada dunia investasi dan keuangan. IHSG sebagai indikator kinerja saham di Indonesia melemah. Saham-saham blue chip sebagai ...pendorong utama bursa mengalami penurunan kinerja. Akan tetapi saham lapis kedua (second liner) berhasil menahan turunnya IHSG sepanjang tahun 2020. Harga rendah dan potensi keuntungan yang tinggi menjadi pertimbangan yang menarik investor beralih pada saham-saham di kelompok tersebut. Saham-saham lapis menengah yang tergabung dalam Index PEFINDO25 cukup mampu mempertahankan kinerja keuangannya. Indikator yang diwakili dengan nilai-nilai rasio keuangan perusahaan cukup baik. Akan tetapi sebagai kelompok saham lapis kedua, investor harus tetap cermat dalam menganalisis kinerja perusahaan-perusahaan tersebut. Pengelompokan perusahaan berdasar karakteristik rasio keuangannya perlu dilakukan. Penelitian ini bertujuan membuat klaster perusahaan yang tergabung dalam Index PEFINDO25 menggunakan Hierarchical Clustering dengan Average Linkage. Jumlah klaster optimum yang diperoleh berdasarkan statistik Pseudo-F adalah 3 klaster. Indikator yang digunakan pada klasterisasi ini adalah rasio keuangan likuditas, solvabolitas, profitabilitas, dan aktivitas data laporan keuangan perusahaan periode tahun 2020 selama masa krisis global. Hasil menunjukkan bahwa KLBF merupakan perusahaan yang mempunyai karakteristik jauh berbeda dibandingkan perusahaan lainnya. Modal kerja yang tinggi disertai rasio keuangan yang baik membuat KLBF menjadi perusahaan terbaik di kelompok ini. BEST, DMAS, MIKA, dan TSPC merupakan kelompok dengan modal kerja sedang dan rasio keuangan baik. Sedangkan AKRA, BULL, ELSA, ERAA, ESSA, GJTL, HEAL, ITMG, LSIP, MDKA, MNCN, PWOR, PTBA, SCMA, SIDO, TBLA, TINS, UNVR, WOOD merupakan klaster perusahaan dengan kriteria modal kerja rendah dan rasio keuangan baik.
Abstract
Aedes aegypti
is a mosquito species that is the main vector of dengue, zika, and chikungunya infection. Indonesia is a tropical country with great potential for transmission of ...Mosquito-Borne Disease. Geo-morphometric analysis is an approach method to evaluate the correlation between shape and the environmental variables that affect to mosquitoes, and to identify variations in mosquito populations by geographical conditions and various types of habitats. The purpose of this study was to determine the geomorphometric differences in the
Ae. aegypti
population from Southeast Aceh District and Sleman District Yogyakarta as well as examining the environmental conditions and factors that might be affecting wings geomorphometry variations. Eggs collection was carried out with ovitraps at both sampling locations. Eggs were hatched till the mature state, at the age of 3rd days, wings preparation is carried out. Digitization and analysis were performed using GM analysis program (Xyom.io). Significant differences (α; p<0.001) were shown in the size and shape of the wings and the distance of mahalanobis of the
Ae. aegypti
from Southeast Aceh and Sleman District. The results revealed that mosquitoes from the two districts are not closely related.
Neonates admitted to neonatal intensive care units are at a risk of developing healthcare-associated infections, leading to increased risk of mortality. This study aimed to identify organisms causing ...such late-onset infections in neonates and determine whether these isolates were genetically identical to those from the surrounding environmental surfaces and hands of healthcare workers (HCWs). A cross-sectional study was carried out over a period of 4 months in a university neonatal intensive care unit (NICU). Samples were collected from all neonates with symptoms of late-onset infections (
n
= 180). Fingerprint samples of 21 healthcare workers as well as 330 random environmental samples were also taken from the unit. Isolates from neonates, environment and fingerprints were subjected to protein electrophoresis followed by sequencing to detect genetic similarities. Almost half of neonatal samples were culture positive (91/180, 50.6%), out of which 72% of bacterial isolates (49/68) were multi-drug resistant.
Klebsiella pneumoniae
(32.6%) and
Candida
spp. (28.4%) were the commonest neonatal isolates. A cluster of two homologous
Klebsiella pneumoniae
strains was isolated from a neonate and an examining bed, while another homologous cluster was from a neonatal sample and a portal incubator. A third cluster was isolated from hands and three neonatal samples. This cluster (caused by
Klebsiella pneumoniae
strain NH54 chromosome) was found to perpetuate over the 4 months of the study. All three clusters were multi-drug-resistant
Klebsiella pneumoniae
. A homologous pair of each of
Candida tropicalis
and
Candida glabrata
was isolated from the blood of two neonates, and one neonatal and a crash cart sample, respectively. Overall, 8.8% (8/91) of neonatal samples were found to be homologous to other neonatal/environmental/hand isolates, denoting perpetuation of pathogens between neonates themselves and also other reservoirs of infections.
Conclusion
: The hands of HCWs, crash carts and incubators are reservoirs of pathogens and can lead to nosocomial infections. Clusters of multi-drug-resistant
Klebsiella pneumoniae
and
Candida
spp. were the predominant neonatal pathogens in this NICU.
What is Known:
• The role of hands and the environment in transmission of infections to neonates is a subject of debate
.
• Genetic sequencing provides solid evidence for detecting homologous strains
.
What is New:
• K. pneumoniae was the most frequently isolated pathogen, and concomitant isolation was found in two cases from the neonatal surroundings (bed/incubator) and hands
.
• Candida spp. with homology were also found in different neonates and environmental samples suggesting risk of transmission
.
Trigonella foenum-graecum L. has an important place among cultivated plants in our country as well as in the world. This study was carried out to determine the morphological and yield properties of ...118 fenugreek genotypes which obtained from
United States Department of Agriculture and local cultivars. Among the genotypes and controls, plant height (24.95-85.15 cm), first pod height (17.00-35.78 cm), pod weight (0.63-63.05 g per plant-1), number of seed per pod (3.56-14.30), pod lenght
(7.01-36.10 cm), 1000 seed weight (0.49-56.31 g) and seed yield per plant (0.21-27.44 g) were determined. As a result of study, the highest seed yield and 1000 seed weight were seen in PI 296394 genotype. Dendogram analysis grouped the set of
fenugreek genotypes into two main groups and many of fenugreek genotypes in same origin were found in different groups.
Nepenthes spp. is a typical plant of Southeast Asia especially Indonesia which has a special leaf modification called a pitcher. The largest number of Nepenthes spp. species in Indonesia is on the ...island of Sumatra. The purpose of this reseach was to identify and analyze cluster Nepenthes spp. from South Sumatra based on morphological characteristics. The specimens were collected from the forest of Tekorejo Village, Air Itam Village and cultivation location in Palembang city of South Sumatra. Identification of morphological characters performed on the characteristics of root, stem, leaves, and pitcher. The morphological data is used for cluster analysis using NTSYS software version 2.02. The identification results showed 9 variants of Nepenthes spp. which belong to the species N. mirabilis, N. gracilis, and N. sumatrana. Dendogram analysis results form two main clusters with a similarity value of 22%. The first cluster consists of N. mirabilis and N. sumatrana. The second cluster consists of N. gracilis. Based on the results of this study can be concluded that the species Nepenthes spp. South Sumatra is N. mirabilis, N. gracilis, and N. sumatrana. The results of this study will be dedicated to updating information about the existence of Nepenthes spp. from South Sumatra and his cluster.
Identifiasi suatu spesies dilakukan dengan cara mengamati berbagai ciri umum maupun khusus dari spesies tersebut berdasarkan karakter morfologi. Tujuan penelitian ini adalah untuk mengidentifiasi dan ...menghitung tingkat kekerabatan Ordo Orthoptera di Hutan Wisata Bromo Karanganyar menggunakan indeks kesamaan Sorensen dan dendogram. Metode penelitian menggunakan deskriptif kuantitatif. Sampel diperoleh melalui sampling di beberapa etape dalam hutan meliputi semak, jalan setapak, sungai dan area pepohonan. Hasil menunjukan bahwa dari 10 spesies yang diperoleh, Melanoplus diffrentialis dan Melanoplus bivitatus memiliki kekerabatan paling dekat yaitu sebesar 92,3% sedangkan kekerabatan paling jauh adalah antara Melanoplus diffrentialis dan Neocurtila hexadactila yaitu sebesar 33,87%. Spesies dalam satu tingkatan takson yang sama cenderung memiliki kemiripan morfologi yang tinggi dibanding dengan spesies dari takson yang berbeda, meski beberapa spesies tidak menunjukkan hal demikian.
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