Rosemary (Rosmarinus officinalis L.), a culinary herb of the family Lamiaceae, has promising anticancer activity. This overview has updated the current knowledge on the chemistry and anticancer ...properties of rosemary extract, carnosic acid, carnosol, and rosmanol, focusing on colon and prostate cancer cells since they are the most susceptible. The information was procured from Google, Google Scholar, PubMed, PubMed Central, Science Direct, J-Stage, and PubChem. Phenolic compounds isolated from the aerial parts of R. officinalis are flavonoids, phenolic acids, diterpenes, triterpenes, terpenoids, and phenylpropanoids. Some of the compounds are new to science, to the genus, and to the species. Almost 30 compounds possess anticancer properties. Rosemary extracts contain abietane diterpenes, with carnosic acid, carnosol, and rosmanol being the most common. Their molecular structures are similar to three fused aromatic rings. Carnosic acid has a –COOH group at C20, carnosol has a lactone ring occurs across the B ring, and rosmanol has a –OH group at C7. Against colon and prostate cancer cells, the rosemary extract and diterpenes inhibited cell viability and induced apoptosis and G2/M phase cell cycle arrest. The inhibition of cell migration and adhesion has also been reported. The rosemary extract and diterpenes also inhibited colon and prostate cancer xenograft in mice. Rosemary extract is more cytotoxic than the diterpenes due to its polyphenols such as flavonoids and triterpenes. In vitro and in vivo cytotoxic activities involve different molecular targets and signalling pathways. Some prospects and areas for future research are suggested.
•RA and CA decrease the formation of fluorescent AGEs and non-fluorescent AGEs such as CML and CEL.•CA decreases the GO and MGO contents, RA decreases only the MGO content in the BSA/Glucose ...model.•RA and CA inhibit the reaction of GO and MGO with BSA.
This work aimed to investigate the effect of the two main components of rosemary extracts, namely rosmarinic acid (RA) and carnosic acid (CA), on the formation of advanced glycation end-products (AGEs) in vitro. In the bovine serum albumin (BSA)/glucose model, addition of RA and CA at 400μg/mL inhibited fluorescent AGEs by more than 90%, and carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) by 82.7% and 75.2%, and 71.4% and 64.2%, respectively. Moreover, the addition of RA and CA at 400μg/mL inhibited fluorescent AGEs by more than 90% both in the BSA/glyoxal (GO) and BSA/methylglyoxal (MGO) models, the formation of CML by 64.9% and 53.9% in BSA/GO model, and CEL by 28.9% and 24.3% in BSA/MGO model, respectively. RA and CA also significantly decreased the concentration of MGO and protein carbonylation.
In dead biological tissues such as human hair, the ability of antioxidants to minimise autoxidation is determined by their chemical reactions with reactive oxygen species. In order to improve our ...understanding of factors determining such antioxidant properties, the mechanistic chemistry of four phenolic antioxidants found in tea and rosemary extracts (epicatechin, epigallocatechin gallate, rosmarinic and carnosic acids) has been investigated. The degradation of
-acetyl alanine by photochemically generated hydroxyl radicals was used as a model system. A relatively high concentration of the antioxidants (0.1 equivalent with respect to the substrate) tested the ability of the antioxidants to intercept both initiating hydroxyl radicals (preventive action) and propagating peroxyl radicals (chain-breaking action). LC-MS data showed the formation of hydroxylated derivatives, quinones and hydroperoxides of the antioxidants. The structure of the assignment was aided by deuterium exchange experiments. Tea polyphenolics (epicatechin and epigallocatechin gallate) outperformed the rosemary compounds in preventing substrate degradation and were particularly effective in capturing the initiating radicals. Carnosic acid was suggested to act mostly as a chain-breaking antioxidant. All of the antioxidants except for rosmarinic acid generated hydroperoxides which was tentatively ascribed to the insufficient lability of the benzylic C-H bond of rosmarinic acid.
Summary
The antioxidant property of rosemary extract as a natural antioxidant on the oxidation process of canola oil has been reported and compared with the synthetic antioxidant butylated ...hydroxytoluene (BHT) (at 200 mg L
ˉ
1
). The effect of free and liposomal encapsulated rosemary extract at concentrations of 200, 600 and 1000 mg L
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1
was evaluated on improving the oxidative stability of canola oil at 120°C using the Rancimat method. The changes in peroxide value, anisidine, TOTOX and thiobarbituric acid (TBA) have been determined by the Schaal oven test at 60°C. Liposomal rosemary extracts were prepared by the Mozafari method. Then physicochemical properties (Z‐potential, particle size and encapsulation efficiency (EE)) were determined. EE and the Z‐potential of liposomes were obtained at 54.59% and −65.1 mV, respectively. The particle size of empty and extract containing liposomes was determined 583.5 nm and 265.4 nm, respectively. The total polyphenol content of the rosemary extract by reagent of the Folin–Ciocalteu's phenol was determined as 164.2 mg gallic acid g
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1
extract. Also, the activity of free radical scavenging of both liposomal encapsulated and free extracts, by the 2‐2‐diphenyl‐1‐picrylhydrazyl (DPPH) method, were 84.57% and 92.5%, respectively. The induction periods of oil containing liposomal rosemary extract at 600, 1000 mg L
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1
and free extract in level 1000 mg L
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1
was higher in comparison with samples containing BHT and blank (
P
≤ 0.05). The incorporation of rosemary extract especially in liposome form into oil delayed the oxidation process. Based on the results of this study, to inhibit oil oxidation, rosemary extract, especially in the liposomal form, can be applied instead of synthetic antioxidants such as BHT.
Summary
Effect of rosemary (Rosmarinus officinalis L.) preparations in baked, vacuum‐packed meatballs from chicken meat during 14 days of storage at +4 °C was assessed. The meatballs were divided ...into the following groups: RD−with 1.0% of dried spice, REE40 and REE70−with 2.0% of ethanol extracts: 40% (v/v) and 70% (v/v), respectively, and REO−with 0.1% of essential oil. The CONTROL product did not contain added rosemary. In chicken meatballs, the antioxidant stability was determined, microbiological analyses as well as sensory evaluation were performed. After 14 days of storage, the strongest antioxidant activity in the chicken meatballs exhibited rosemary ethanol extracts. Compared with the CONTROL, the REE70 and REO had the best microbiological quality at the end of the storage period. All products with rosemary were sensory acceptable; however, the highest scores in the evaluation of all sensory attributes were obtained by the RD treatment.
Chicken thigh meat, mechanically desinewed meat from chickens and pork jowl were used as raw materials to produce meatballs with various rosemary preparations. The rosemary preparations added to chicken meatballs were: dried rosemary, ethanol extracts from rosemary and rosemary essential oil. The results show that rosemary, in various forms, possesses antioxidant and antimicrobial properties and the use of this herb is a promising strategy for maintaining the key quality characteristics of ready‐to‐eat chicken meat products prepared from mechanically desinewed chicken meat.
We previously performed a 4 week interventional trial that suggested that continuous intake of rosemary extract improves the mood states, fatigue, and cognitive function of working generation healthy ...adult Japanese men. However, the severity of depression in participants in our previous study was relatively mild. Therefore, in the present study, a post-hoc analysis of our previous study was conducted, limited to participants whose total mood disturbance (TMD) scores, which indicate greater mood disturbance, were above the median at baseline, to evaluate whether rosemary extract was effective for individuals with poor mental health. Following the intervention, the scores of TMD and "Confusion-Bewilderment" were significantly decreased (both
< 0.05), and scores of "Vigor-Activity" were significantly increased in the rosemary group (
= 8) compared with those in the control group (
= 13;
< 0.01). When comparing the scores from pre- and post-intervention, significant improvements in "Tension-Anxiety", "Vigor-Activity", "Fatigue on awakening", "Daytime sleepiness", and "Psychomotor speed" were observed in the rosemary group only (all
< 0.05). Based on these results, it was expected that rosemary extracts were effective for improving the mental energy and sleep quality of work-age men with poor mental health.
This study investigated within-plant variability of the main bioactive compounds in rosemary (
L.). Volatile terpenes, including the enantiomeric distribution of monoterpenes, and phenols were ...analyzed in young and mature foliar, cortical and xylem tissues. In addition, antimicrobial activity of rosmarinic acid and selected terpenes was evaluated against two rosemary pathogens,
and
. Data showed that total concentration and relative contents of terpenes changed in relation to tissue source and age. Their highest total concentration was observed in the young leaves, followed by mature leaves, cortical and xylem tissues. Rosmarinic acid and carnosic acid contents did not show significant differences between leaf tissues of different ages, while young and mature samples showed variations in the content of four flavonoids. These results are useful for a more targeted harvesting of rosemary plants, in order to produce high-quality essential oils and phenolic extracts. Microbial tests showed that several terpenes and rosmarinic acid significantly inhibited the growth of typical rosemary pathogens. Overall, results on antimicrobial activity suggest the potential application of these natural compounds as biochemical markers in breeding programs aimed to select new chemotypes less susceptible to pathogen attacks, and as eco-friendly chemical alternatives to synthetic pesticides.
Antioxidants are used to prevent the oxidation of foods. When used for food additive purposes, the dosage should be regulated and the functionality evaluated to ensure stability. In this study, we ...performed a method validation for the quantitative analysis of rosemary extract residues and evaluated the antioxidant activity of rosemary extract in food matrices. The validated method was able to determine rosemary extract under the optimized high-performance liquid chromatography-photodiode array (HPLC-PDA) conditions. Furthermore, the antioxidant activity was evaluated by peroxide value, acid value, and in terms of the residual antioxidant levels in lard oil. For HPLC-PDA analysis, the limit of detection and quantification of rosemary extracts was ranged from 0.22 to 1.73 μg/mL, 0.66 to 5.23 μg/mL and the recoveries of the rosemary extracts ranged from 70.6 to 114.0%, with relative standard deviations of between 0.2% and 3.8%. In terms of antioxidant activity, carnosic acid performed better than carnosol. Furthermore, by evaluation of the residual antioxidant level using HPLC, we found that carnosic acid is more stable in lard oil than carnosol. These results indicate that rosemary extract can be used as an antioxidant and that the analytical method is suitable for the determination of rosemary extract in various food samples.
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