The research investigated the vegetative propagation of Bagoadlau (Xanthosthemon philippinensis Merr.) stem cuttings using different concentrations of indolebutyric acid (IBA). Pretreated cuttings ...were planted in polyethylene bags and observed in a fully covered chamber for 45 days. The study employed a single trial of completely randomized design (CRD) with five treatments (500 ppm, 100 ppm, 1,500 ppm, and 2000 ppm), each replicated four times. Results indicated significant variations among treatment means in terms of percent shooting, shoot length, percent rooting, number and length of adventitious roots, and percent callusing. Notably, 500 ppm and 1,500 ppm demonstrated the highest outcomes in percent shooting, shoot length, percent survival, measurement of adventitious roots, count of roots, and percentage of rooting. In contrast, the survival percentage of T2 (500 ppm) displayed the highest rate and consistent performance compared to other treatments. Hence, the study recommends using 500 ppm and 1,500 ppm for effective rooting of Bagoadlau (Xanthosthemon philippinensis Merr.) stem cuttings. The successful propagation of native species like Bagoadlau is particularly relevant to ecosystem restoration efforts in regions marked by land degradation and mining activities. The study addresses several crucial aspects by successfully establishing Bagoadlau on such lands. This includes the restoration of native biodiversity, improved soil, and water quality through stabilizing soil, preventing erosion, and acting as a natural filtration system.
Background: The sexual propagation of Cocoa (Theobroma cacao L.) has some limitations to preserve some desirable agronomic characteristics in successive generations. Objective: The present research ...evaluates the effect of a chitosan based-formulation (Quitomax®) on morpho-physiological, rooting attributes and benefit-cost ratio of two cocoa varieties during vegetative propagation. Methodology: The experimental design was completely randomized with a factorial arrangement (A × B), where A represented the two clones and B the three concentrations of chitosan based formulation used (0, 100, 500 and 1000 mg L-1), with three repetitions. per treatment. The survival (%), the stem diameter (mm), the number of leaves, the number and length (cm) of roots, the biomass (g), the gas exchange (A, gs, Ci, E), and an economic analysis of the two cocoa plant varieties were evaluated at 120 days after starting the trial. Results: The clone CCN-51 treated with 500 mg L-1 had significantly the highest results on survival (80%), stem diameter (6.83 mm), number of leaves per plant (8.2), number and length of roots (6.21 and 35.74 cm), aerial and root biomass (4.07 g and 1.64 g) parameters. In gas exchange, the highest values of Water use efficiency (WUE) were observed at 500 mg L-1 in CCN-51 (5.36 mmol mol-1) and 1000 mg L-1 in INIAP-EETP-801 (7.62 mmol µmol-1). In both clones, higher profitability was obtained when applying the chitosan dose of 500 mg L-1, reaching profitability of 40.65 and 50.00% for clones INIAP-EETP-801 and CCN-51, respectively. Implications: The cocoa clone CCN-51 showed plants that exhibited greater development of both the aerial part and the root part of the cocoa seedlings than INIP-EETP-801 coca clones. Conclusion: The chitosan based formulation at 500 mg L-1 is a promissory alternative to improve the evaluated parameters after 120 days of cocoa vegetative propagation.
In clonal forestry, eucalypts are propagated by stem cuttings on a large scale. Six-month-old seedlings of 20 seedlots of Eucalyptus camaldulensis were propagated by stem cuttings and segregated ...according to their pre-harvest position (apical, mid-position and apical) in the parent shoot. 'Rooted (%)' - the number of cuttings rooted as a percentage of the original number - was compared to climatic data from near the sites of seed collection. Seedlot mean rooted (%) tended to vary with the warmth of the native climate, suggesting that warm-climate seedlots were less well-adapted to the cool conditions that prevailed. Apical cuttings had the highest rooted (%) compared to mid-position and basal cuttings, suggesting variation in the unrooted survival of cuttings rather than in the rooting ability of the survivors. The results indicate the likelihood of seedlot
propagation system interactions in E. camaldulensis when propagated by stem cuttings.
Abstract Magnolia biondii Pamp is an important ornamental tree species widely grown and used as a rootstock in the propagation of different Magnolia varieties. In the current studies, anatomical, ...physiological and endogenous hormones were studied to check the effect of IBA 750 mg/L on the adventitious rooting and to provide theoretical and technical support for the propagation of Magnolia biondii Pamp through stem cuttings. Two thousand stem cuttings were prepared and divided into two groups i.e., IBA treated cuttings and water control. For the evaluation of antioxidant enzyme activities, and endogenous hormones levels, samples were collected on the day of planting and each 5th day and further steps were carried out in the laboratory according to the protocols and proper precautions. For the anatomical observations, samples were collected on the 13th, 15th, and 17th day for IBA treated cuttings while 21st, 23rd, and 25th day for control. Collected samples were preserved in the FAA solution and further observations were carried out in the laboratory. Anatomical observations showed that it took 13 days for the differentiation of root primordia to the appearance of young adventitious roots in IBA treated cuttings, while it took 21 days to develop primordia in the control. Antioxidant enzyme activities involved in ROS were significantly higher in the IBA treated cuttings compared to control. POD showed a peak on the 13th day before the emergence of roots in IBA treated cuttings while it showed a peak on the 21st day in the control. PPO showed a peak on the 21st day in the IBA treated cuttings while it showed a peak on the 29th day in the control. SOD showed a peak on the 17th day in IBA treated cuttings, while it showed a peak on the 25th day in the control. Exogenous application of IBA enhanced the endogenous IAA and GA3 levels compared to CK, while it reduced the levels of ABA continuously at the time of rooting and then increased gradually. Inclusively, our study suggests that IBA 750 mg/L is efficient for the rooting of Magnolia biondii Pamp cuttings, as it enhanced the process of antioxidant enzyme activities, endogenous hormones levels and reduced the time of root formation which is evident from the anatomical observations.
Resumo Magnolia biondii Pamp é uma importante espécie de árvore ornamental muito cultivada e utilizada como porta-enxerto na propagação de diferentes variedades de Magnolia. Nos estudos atuais, hormônios anatômicos, fisiológicos e endógenos foram estudados para verificar o efeito do AIB na dose de 750 mg / L no enraizamento adventício e fornecer suporte teórico e técnico para a propagação de M. biondii Pamp por meio de estacas. Duas mil estacas foram preparadas e divididas em dois grupos, ou seja, tratadas com AIB e controle de água. Para a avaliação das atividades das enzimas antioxidantes e dos níveis de hormônios endógenos, as amostras foram coletadas no dia do plantio e a cada 5 dias, enquanto as demais etapas foram realizadas em laboratório de acordo com os protocolos e os devidos cuidados. Para as observações anatômicas, as amostras foram coletadas no 13º, 15º e 17º dias para estacas tratadas com AIB e no 21º, 23º e 25º dias para o controle. As amostras coletadas foram preservadas em solução FAA, e outras observações foram realizadas em laboratório. Observações anatômicas mostraram a necessidade de 13 dias para a diferenciação dos primórdios radiculares até o aparecimento de raízes adventícias jovens em estacas tratadas com AIB e de 21 dias para o desenvolvimento dos primórdios no controle. As atividades das enzimas antioxidantes envolvidas nas ROS foram significativamente maiores nas estacas tratadas com AIB em comparação com o controle. A POD apresentou pico no 13º dia antes da emergência das raízes nas estacas tratadas com AIB, enquanto no 21º dia apresentou pico no controle. A PPO teve pico no 21º dia nas estacas tratadas com AIB e no 29º dia no controle. A SOD apresentou pico no 17º dia nas estacas tratadas com AIB e no 25º dia no controle. A aplicação exógena de AIB aumentou os níveis endógenos de IAA e GA3 em relação ao controle, enquanto reduziu os níveis de ABA continuamente no momento do enraizamento e, em seguida, aumentou gradativamente. Inclusive, nosso estudo sugere que o AIB na dose de 750 mg / L é eficiente para o enraizamento de estacas de M. biondii Pamp, visto que potencializou o processo de atividades de enzimas antioxidantes e os níveis de hormônios endógenos, além de reduzir o tempo de formação de raízes, o que fica evidente nas observações anatômicas.
This study was conducted in the botanical garden, Department of biology, College of Science/ Mustansiriyah University in from (15 February to 15 March, 2019) under the natural environmental ...conditions in the greenhouse in order to evaluate the effectiveness of parsley aqueous extract as a promoter for rooting. The study included the use of aqueous extract of a plant Parsley (Petroselinum crispum) extract was used in concentrations (1.25, 2.5 g / l), compare with IBA in concentration (100 mg / L) with dipping time 24 hour for all treatments. The cutting stems were included Rosmarinus officinalis, Nerium oleander, Olea europaea, Plumeria alba, Hibiscus rosa, Pelargonium graveolens, and Myrtus communis. The following measurements were taken after 30 days from the beginning of the experiment: rooting percentage, (number and length of roots), number of new (leaves and branches). Plant hormone concentrations, oxidative enzyme activity (SOD, POS) and amino acids were also analyzed for the extract that gave the highest values for all traits. The results showed that there were significant differences between treatments, and indicated that parsley extract in the concentration (2.5 g / L) was more exceeded compared with other treatments. The chemical analysis of parsley extract showed the presence of the hormones IAA, GA3, cytokinin and ABA as well as the highest activity of the antioxidant enzymes (POS and SOD), also the analysis of parsley indicated the presence of several types of amino acids, including Tryptophan, Glutamic acid, alanine, valine, Tyrosine, Phenylalanine Histadine, Ornithine, Arginine and other amino acids. In conclusion, the Parsley extract can be used as promoter rooting for stem cuttings as a new method instead of using plant growth regulators , Replace the IBA which used in the rooting of stem cuttings by some natural extracts which can stimulate the rooting process.
Abstract
Recalcitrant adventitious root (AR) development is a major hurdle in propagating commercially important woody plants. Although significant progress has been made to identify genes involved ...in subsequent steps of AR development, the molecular basis of differences in apparent recalcitrance to form AR between easy-to-root and difficult-to-root genotypes remains unknown. To address this, we generated cambium tissue-specific transcriptomic data from stem cuttings of hybrid aspen, T89 (difficult-to-root) and hybrid poplar OP42 (easy-to-root), and used transgenic approaches to verify the role of several transcription factors in the control of adventitious rooting. Increased peroxidase activity was positively correlated with better rooting. We found differentially expressed genes encoding reactive oxygen species scavenging proteins to be enriched in OP42 compared with T89. A greater number of differentially expressed transcription factors in cambium cells of OP42 compared with T89 was revealed by a more intense transcriptional reprograming in the former. PtMYC2, a potential negative regulator, was less expressed in OP42 compared with T89. Using transgenic approaches, we demonstrated that PttARF17.1 and PttMYC2.1 negatively regulate adventitious rooting. Our results provide insights into the molecular basis of genotypic differences in AR and implicate differential expression of the master regulator MYC2 as a critical player in this process.
Comparative transcriptomic analysis from the cambium of two Populusgenotypes with contrasting adventitious rooting phenotype reveals higher transcriptional activity in the easy-to-root compared with the difficult-to-root genotype.
Background and objective: Kirengeshoma koreana Nakai is an endemic and endangered species in South Korea. We conducted in vitro propagation and regeneration of K. koreana from stem cuttings to ...investigate the effects of nine different basal culture media and five different carbohydrate sources on its growth.Methods: Apical segments (at least 1 cm long) collected randomly from a six-week-old K. koreana plantlet grown in vitro were used as explants. In the first experiment, the explants were transferred into square vessels containing 50 mL of nine different basal culture media supplemented with 30 g⋅L-1 sucrose and 3 g⋅L-1 Phytagel. In the second experiment, the explants were transferred into square vessels containing 50 mL half-strength SH medium supplemented with five different carbohydrate sources at 30 g⋅L-1. Each medium was solidified with 3 g⋅L-1 Phytagel. All experiments contained 4 cultures, and the experiments were repeated four times to enhance reproducibility. Data on stem length, shoot fresh weight, leaf width, leaf length, root count, and root length were collected at the end of 8 weeks of culture.Results: ANOVA showed that the basal culture medium had a significant effect on K. koreana growth (p < .001). The half-strength SH medium was the best condition for stem length, shoot fresh weight, leaf width and root length (3.76 ± 0.12 cm, 0.60 ± 0.06 g, 1.19 ± 0.05 cm, and 2.83 ± 0.13 cm, respectively). However, the highest percentage increase in root count (13.00 ± 0.90) was found to occur with half-strength WPM. The effect of different carbohydrate sources on K. koreana growth was significantly different (p < .001), with the exception of stem length and leaf width (p = .26 and p = .09, respectively). Maltose was the best condition for shoot fresh weight (0.90 ± 0.09 g). Although there was no significant difference, sucrose was found to be best for leaf width, leaf length and root length (1.34 ± 0.07 cm, 2.34 ± 0.10 cm, and 3.86 ± 0.19 cm, respectively).Conclusion: This in vitro propagation and regeneration system for K. koreana shows promise in terms of scalability and could help greatly with germplasm conservation and restoration efforts for the species.
One of the determining factors for the success of red betel (Piper Crocatum Ruiz) stem cuttings is the growing medium used to grow roots and shoots. This study aims to understand the influence of the ...type of planting media on the growth of roots, shoots, and height of red betel seedlings and to determine the best planting media that supports the growth of red betel roots and shoots. The study was conducted through an experiment using a Randomized Block Design, consisting of 6 types of planting media and each treatment was repeated four times. The treatments were 1. Sand, 2. Soil, 3. Husk biochar, 4. Soil + Husk biochar, 5. Soil + Sand, 6. Husk biochar + Sand. The research data were analyzed using Analysis of Variance and continued with Duncan Multiple Range Test at 5% level. The results showed that the type of media for growing red betel stem cuttings had an effect on root length, number of shoots, available N. The type of planting media that best supports the growth of roots, shoots and plant height of red betel stem cuttings is husk biochar planting media. Keywords: Planting media, Red betel, Stem cuttings, Root growth, Plant height.
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