A tomato core collection consisting of 122 gene bank accessions, including landraces, old cultivars, and wild relatives, was explored for variation in several plant growth, yield and fruit quality ...traits. The resequenced accessions were also genotyped with respect to a number of mutations or variations in key genes known to underlie these traits. The yield-related traits fruit number and fruit weight were much higher in cultivated varieties when compared to wild accessions, while, in wild tomato accessions, Brix was higher than in cultivated varieties. Known mutations in fruit size and shape genes could well explain the fruit size variation, and fruit colour variation could be well explained by known mutations in key genes of the carotenoid and flavonoid pathway. The presence and phenotype of several plant architecture affecting mutations, such as self-pruning (sp), compound inflorescence (s), jointless-2 (j-2), and potato leaf (c) were also confirmed. This study provides valuable phenotypic information on important plant growth- and quality-related traits in this collection. The allelic distribution of known genes that underlie these traits provides insight into the role and importance of these genes in tomato domestication and breeding. This resource can be used to support (precision) breeding strategies for tomato crop improvement.
Background: Tomato brown rugose fruit virus (ToBRFV) has spread worldwide affecting tomato crop production in several countries. Although there are commercial ToBRFV-resistant tomato varieties; ...still, careful monitoring of its prevalence is necessary for establishing feasible epidemiological control programs. Since Sonora has a strategic geographic location for tomato cultivation and export, studies on the detection and prevention of ToBRFV outbreaks are pertinent as part of the actions to achieve the eradication of this virus. Objective: To determine the recent prevalence of ToBRFV in tomato-producing areas of the state of Sonora, Mexico in the period 2021-2022, using a combination of molecular and serological tools. Methodology: Samples of tomato leaves and fruits were collected from commercial greenhouses in different regions of Sonora from September 2021 to June 2022. Forty-four tomato leaves and 42 seed samples were analyzed for ToBRFV using reverse transcriptase-polymerase chain reaction and by direct detection of viral epitopes by immunochromatography. Results: 60% and 48% of the samples tested positive for ToBRFV by immunochromatography and RT-qPCR, respectively, showing an exact agreement regarding the samples’ origin. Implications: Future research may focus on genomic analysis of viral isolates that would provide clues of ToBRFV propagation speed and emergence of particular viral strains typical of the place of origin. Conclusion: The presence of ToBRFV in the state of Sonora, Mexico is limited to specific geographic sites, which suggests that local regulations and phytosanitary actions have worked to control the spread of the virus.
The scents of volatile organic compounds (VOCs) are an important component of ripe fleshy fruit attractiveness, yet their variation across closely related wild species is poorly understood. ...Phylogenetic patterns in these compounds and their biochemical pathways offer insight into the evolutionary drivers of fruit diversity, including whether scent can communicate an honest signal of nutrient content to animal dispersers. We assessed ripe fruit VOC content across the tomato clade (Solanum sect. Lycopersicon), with implications for crop improvement since these compounds are key components of tomato flavor.
We analyzed ripe fruit volatiles from 13 species of wild tomato grown under common garden conditions. Interspecific variation in 66 compounds and their biochemical pathways was assessed in 32 accessions, with an accession-level phylogeny accounting for relatedness.
Wild tomato species can be differentiated by their VOCs, with Solanum pennellii notably distinct. Phylogenetic conservatism exists to a limited extent. Major clade-wide patterns corresponded to divergence of the five colored-fruited species from the nine green-fruited species, particularly for nitrogen-containing compounds (higher in colored-fruited) and esters (higher in green-fruited), the latter appearing to signal a sugar reward.
We established a framework for fruit scent evolution studies in a crop wild relative system, showing that each species in the tomato clade has a unique VOC profile. Differences between color groups align with fruit syndromes that could be driven by selection from frugivores. The evolution of colored fruits was accompanied by changes in biochemical pathways underlying esters and nitrogen-containing compounds, volatiles important to tomato flavor. This article is protected by copyright. All rights reserved.
Summary
Carotenoids are vital phytonutrients widely recognised for their health benefits. Therefore, it is vital to thoroughly investigate the metabolic regulatory network underlying carotenoid ...biosynthesis and accumulation to open new leads towards improving their contents in vegetables and crops.
The outcome of our study defines SlWRKY35 as a positive regulator of carotenoid biosynthesis in tomato. SlWRKY35 can directly activate the expression of the 1‐deoxy‐d‐xylulose 5‐phosphate synthase (SlDXS1) gene to reprogramme metabolism towards the 2‐C‐methyl‐d‐erythritol 4‐phosphate (MEP) pathway, leading to enhanced carotenoid accumulation. We also show that the master regulator SlRIN directly regulates the expression of SlWRKY35 during tomato fruit ripening.
Compared with the SlLCYE overexpression lines, coexpression of SlWRKY35 and SlLCYE can further enhance lutein production in transgenic tomato fruit, indicating that SlWRKY35 represents a potential target towards designing innovative metabolic engineering strategies for carotenoid derivatives.
In addition to providing new insights into the metabolic regulatory network associated with tomato fruit ripening, our data define a new tool for improving fruit content in specific carotenoid compounds.
The production of tomatoes has experienced a huge rise over the years as a result of its economic, nutritional and cancer reduction importance. Despite the rapid advancement of technology in the past ...century, storage of tomato fruits remains a major problem experienced in the postharvest chain in most developing countries. This study gives a survey of the various causes of tomatoes postharvest losses, the different methods used in storage of the fruit over time and their limitations. It was found that the conventional methods used in tomatoes storage, improved its shelf life but was accompanied by some significant losses in quantity and quality. Hence, generating a need for a postharvest storage system taking into cognizance the optimum conditions required for the fruit storage. The development of a thermally controlled postharvest storage system with the sole purpose of increasing shelf life and minimize the rate of deterioration becomes inevitable.
Abiotic stresses due to environmental factors could adversely affect the growth and development of crops. Among the abiotic stresses, drought and heat stress are two critical threats to crop growth ...and sustainable agriculture worldwide. Considering global climate change, incidence of combined drought and heat stress is likely to increase. The aim of this study was to shed light on plant growth performance and leaf physiology of three tomatoes cultivars ('Arvento', 'LA1994' and 'LA2093') under control, drought, heat and combined stress.
Shoot fresh and dry weight, leaf area and relative water content of all cultivars significantly decreased under drought and combined stress as compared to control. The net photosynthesis and starch content were significantly lower under drought and combined stress than control in the three cultivars. Stomata and pore length of the three cultivars significantly decreased under drought and combined stress as compared to control. The tomato 'Arvento' was more affected by heat stress than 'LA1994' and 'LA2093' due to significant decreases in shoot dry weight, chlorophyll a and carotenoid content, starch content and NPQ (non-photochemical quenching) only in 'Arvento' under heat treatment. By comparison, the two heat-tolerant tomatoes were more affected by drought stress compared to 'Arvento' as shown by small stomatal and pore area, decreased sucrose content, Φ
(quantum yield of photosystem II), ETR (electron transport rate) and q
(fraction of open PSII centers) in 'LA1994' and 'LA2093'. The three cultivars showed similar response when subjected to the combination of drought and heat stress as shown by most physiological parameters, even though only 'LA1994' and 'LA2093' showed decreased F
/F
(maximum potential quantum efficiency of photosystem II), Φ
, ETR and q
under combined stress.
The cultivars differing in heat sensitivity did not show difference in the combined stress sensitivity, indicating that selection for tomatoes with combined stress tolerance might not be correlated with the single stress tolerance. In this study, drought stress had a predominant effect on tomato over heat stress, which explained why simultaneous application of heat and drought revealed similar physiological responses to the drought stress. These results will uncover the difference and linkage between the physiological response of tomatoes to drought, heat and combined stress and be important for the selection and breeding of tolerant tomato cultivars under single and combine stress.
Tomato sucker or axillary shoots should be removed to increase the yield and reduce the disease on tomato plants. It is an essential step in the tomato plant care process. It is usually performed ...manually by farmers. An automated approach can save a lot of time and labor. In the literature review, we see that semantic segmentation is a process of recognizing or classifying each pixel in an image, and it can help machines recognize and localize tomato suckers. This paper proposes a semantic segmentation neural network that can detect tomato suckers quickly by the tomato plant images. We choose RGB-D images which capture not only the visual of objects but also the distance information from objects to the camera. We make a tomato RGB-D image dataset for training and evaluating the proposed neural network. The proposed semantic segmentation neural network can run in real-time at 138.2 frames per second. Its number of parameters is 680, 760, much smaller than other semantic segmentation neural networks. It can correctly detect suckers at 80.2%. It requires low system resources and is suitable for the tomato dataset. We compare it to other popular non-real-time and real-time networks on the accuracy, time of execution, and sucker detection to prove its better performance.
Tomato peel is an industrial waste product from tomato vegetables that has not been utilized properly. Industrial waste that has not been utilized will harm the environment. Tomato peel is a source ...of natural food coloring, antioxidants, and a source of fiber. Application of tomato peel can be added to mayonnaise products to improve the color of low-fat mayonnaise. This study aimed to investigate the effect of giving tomato peel nanopowder as a natural coloring in low-fat mayonnaise based on pH, total acid, protein content, carbohydrates, color, and sensory quality. Low-fat mayonnaise was prepared using sunflower oil, vinegar, and egg yolks by giving the tomato peel nanopowder treatment of 0, 1, 2, and 3% which was repeated 5 times. Analysis of variance (ANOVA) was used as data analysis if there were differences in effect between the treatments, followed by Duncan's Multiple Range Test (DMRT). The research results of adding tomato peel nanopowder with different percentages had a very significant effect (p<0.01) on pH, total acid, protein content, carbohydrates, color, and sensory quality. This research concludes that 3% tomato peel nanopowder applied to low-fat mayonnaise can improve pH, total acid, protein content, carbohydrate, L, a*, b*, and sensory quality.
Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is among the most devastating pests worldwide of solanaceous plants, particularly of tomato (Solanum lycopersicum L.). Since its recent introduction ...into sub‐Saharan Africa, it has become an additional widespread tomato pest throughout the region. When no control measures are taken, yield losses caused by T. absoluta larvae can reach 100%. Chemical control remains the primary management option despite its many adverse effects and increasing inefficiency due to the species’ endophytic habits and ability to develop insecticide resistance. Safer and more effective alternatives are therefore urgently required. Here, we investigated the effectiveness of two bioinsecticides compared to that of a widely used deltamethrin‐based synthetic insecticide, in preventing damage caused by T. absoluta larvae to tomato leaves and fruits, thereby affecting tomato yield. A field trial was conducted in 2018 and 2019 in northwestern Senegal using a randomized complete block design. Damage caused by T. absoluta affected approximately 65% of total leaf area and 3–24% of the fruits. Damage to the leaves caused by other insect species or other factors was less significant. At the dose applied, the chemical insecticide did not significantly reduce the surface area of leaflets affected by T. absoluta or other causal agents, the number of T. absoluta mines on the leaflets, or the proportion of fruits damaged. In contrast, the azadirachtin and Bacillus thuringiensis (Bt) formulations rapidly and significantly reduced damage to both leaves and fruits. The proportion of fruits damaged was reduced by a factor two and the total tomato yield was improved, particularly when the Bt formulation was used. In addition to their effectiveness in reducing damage caused by T. absoluta larvae to tomato leaves and fruit, and increasing the yield of marketable fruits, the viability of these organic alternatives was shown. The scope of our results is discussed in the context of T. absoluta control, in particular in integrated pest management programmes for tomato crops.
Tuta absoluta (Lepidoptera: Gelechiidae) is among the most devastating pests worldwide of solanaceous plants, particularly of tomato, Solanum lycopersicum. Here, the effectiveness of two bioinsecticides – azadirachtin and Bacillus thuringiensis (Bt) formulations – was demonstrated as viable alternatives to the widely used deltamethrin‐based chemical insecticide in reducing damage caused by T. absoluta larvae to tomato leaves and fruits, and thus increasing the yield of marketable fruits in northwestern Senegal.
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