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Wenegieme, Tara-Yesomi; Elased, Dalia; McMichael, Kelia E; Rockwood, Jananie; Hasrat, Khanzada; Ume, Adaku C; Marshall, Andrea G; Neikirk, Kit; Kirabo, Annet; Elased, Khalid M; Hinton, Antentor; Williams, Clintoria R
American journal of physiology. Heart and circulatory physiology, 2024-Jun-01, Letnik: 326, Številka: 6Journal Article
Given the growing interest in the role of zinc in the onset and progression of diseases, there is a crucial demand for reliable methods to modulate zinc homeostasis. Using a dietary approach, we provide validated strategies to alter whole-body zinc in mice, applicable across species. For confirmation of zinc status, animal growth rates as well as plasma and urine zinc levels were evaluated. The accessible and cost-effective methodology outlined will increase scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of diseases. This methods paper details dietary approaches to alter zinc homeostasis in rodents and qualitative and quantitative methods to ensure the zinc status of experimental animals. The outlined accessible and cost-effective protocol will elevate scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of a multitude of health conditions and diseases.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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