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Dogandzhiyski, Plamena; Ghidini, Alice; Danneberg, Friederike; Strömberg, Roger; Göbel, Michael W
Bioconjugate chemistry, 12/2015, Letnik: 26, Številka: 12Journal Article
A new peptide nucleic acid (PNA) construct carrying a tris(2-aminobenzimidazole) phosphodiester cleaver is presented. This non-metal-based artificial nuclease hydrolyzes RNA substrates that form a bulge upon binding to the PNA. Reaction rates depend on the bulge sequence. For conjugates of tris(2-aminobenzimidazole), substrate turnover is shown for the first time. Two methods of analysis for the kinetics are compared: IE-HPLC separation of oligonucleotide fragments and analysis of Cy5-labeled oligonucleotide fragments by denaturating PAGE on a DNA sequencer, respectively. The different methods give rates that are in the same range where, in general, the substrates for the sequencer method give slightly lower rates.
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| Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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| JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP | |
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