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Marikar, Faiz M.M.T. (Nanjing University, Nanjing, P.R. China); Fang, Lei (Nanjing University, Nanjing, P.R. China); Jiang, Shu-Han (Nanjing University, Nanjing, P.R. China); Hua, Zi-Chun (Nanjing University, Nanjing, P.R. China), E-mail: zchua@nju.edu.cn
Journal of microbiology and biotechnology, 05/2007, Letnik: 17, Številka: 5Journal Article
In the present article, a novel fusion expression vector for Escherichia coli was developed based on the pTORG plasmid, a derivative of pET32a. This vector, named pT7MT (GenBank Accession No DQ504436), carries a T7 promoter and it drives the downstream gene encoding Metallothionein 2A (MT2A). There are in-framed multiple cloning sites (MCS) downstream of the MT2A gene. A target gene can be cloned into the MCS and fused to the C-terminal of the MT2A gene in a compatible open reading frame (ORF) to achieve fusion expression.
