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Davies, James O J; Telenius, Jelena M; McGowan, Simon J; Roberts, Nigel A; Taylor, Stephen; Higgs, Douglas R; Hughes, Jim R
Nature methods, 01/2016, Letnik: 13, Številka: 1Journal Article
Methods for analyzing chromosome conformation in mammalian cells are either low resolution or low throughput and are technically challenging. In next-generation (NG) Capture-C, we have redesigned the Capture-C method to achieve unprecedented levels of sensitivity and reproducibility. NG Capture-C can be used to analyze many genetic loci and samples simultaneously. High-resolution data can be produced with as few as 100,000 cells, and single-nucleotide polymorphisms can be used to generate allele-specific tracks. The method is straightforward to perform and should greatly facilitate the investigation of many questions related to gene regulation as well as the functional dissection of traits examined in genome-wide association studies.
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