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Schofield, Jeremy A; Duffy, Erin E; Kiefer, Lea; Sullivan, Meaghan C; Simon, Matthew D
Nature methods, 03/2018, Letnik: 15, Številka: 3Journal Article
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.
