Wu, Jianglai; Liang, Yajie; Chen, Shuo; Hsu, Ching-Lung; Chavarha, Mariya; Evans, Stephen W; Shi, Dongqing; Lin, Michael Z; Tsia, Kevin K; Ji, Na
Nature methods,
03/2020, Letnik:
17, Številka:
3
Journal Article
Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.
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