The study shows the role of biochemical variables, which be developed as training adaptation for training program or what is called the internal load adaptation to outer load, and quantifies in the training adaptation and recovery rate after sport achievement using anaerobic training program that aims at developing the speed endurance of sprint runners. The study aims to show the followings: 1-        Significant differences between the pre and post tests of the functional variables directly  after anaerobic exercise and recovery. The researchers used the experimental method via the experimental design of pre and post test. The research Sample included (6) male athlete of short distance runners whom they were represent Nineveh athletic team for track and field. The sample was intentionally chosen. Tests, measurements, technical devices and scientific references were used as data collection methods and means. For statistical treatments, the researcher used Mean, Standard Deviation, t. Test. changes% ratio. Conclusions: 1-   The training program resulted in an increase in the systolic blood pressure and decrease in the diastolic blood pressure between the pre and post immediately after the anaerobic effort for the favor of post test. 2-   Regadles of using low intensity interval training in the suggested anaerobic training program in speed endurance, but it results in observed difference in the performance of Wingate test.   Recommendations: 1-   Coaches should concentrate on training programs that aims at developing the speed endurance in preparatory and special period of training for short distance runners according to proper scientific basics, taking into consideration using the components of training load that correspond with the abilities and capabilities of athletes.