This work aimed to focus on the antibacterial properties of garlic nanoemulsion on some multidrug resistance (MDR) strains of Pseudomonas aeruginosaisolated from broiler farms and hatcheries in Sharkia and Ismailia governorates, Egypt. Pseudomonas spp. was isolated in 21.3% of collected samples. It was isolated from younger broilers 1-10 days with an incidence rate of 22% (11/50), older broilers 16% (8/50), dead embryo in shell 31.4% (11/35), and from hatcheries was 13.3% (2/15). There was a variable range of antibiotic resistance ranging from 66.7-100% against the isolated strains of P. aeruginosa. Tetracycline and sulfamethoxazole-trimethoprim achieved the highest resistance rates, while penicillin and gentamycin were of a lower rate. However, norfloxacin, ciprofloxacin, and colistin were the most sensitive antibiotics against examined MDR P. aeruginosa.16SrDNA gene was found in ten P. aeruginosa isolates. Theseisolates were found to be virulent as oprL gene was detected in all isolates 100%. In addition, tetA(A),blaTEM,arr, and mexRantibiotic resistance genes were shown positive 100% in all MDR P. aeruginosa isolates. Minimum Inhibitory Concentration (MIC) values showed that garlic nanoemulsion (GN) was effective against examined P. aeruginosa at different concentrations. GN had 29.61% sulfur compounds of active components with 0.52 ug/ml of IC50 and 40.94 nm size with polydispersity index: 0.165 using dynamic light scattering had a 19.6± 5.11mV. In conclusion, the application of garlic nanoemulsion is an excellent alternative candidate to antibiotics for treatment because it significantly reduced the gene expression levels of MDR P. aeruginosa in broiler farms.