In situ electron density measurements by the CHAllenging Minisatellite Payload and the Defense Meteorological Satellites Program F17 satellites show that the midlatitude ionization at altitudes of ∼350 and 850 km is enhanced in the late evening. The enhancements increase to maximum around midnight and are clearly observed till early morning as the equatorial ionization decays to minimal level. They appear in the winter hemisphere during June and December solstices and in both hemispheres during equinox. The enhancements are well confined between ±30° and ±50° magnetic latitude, with the magnetic flux tubes of L = 1.3 − 2.4 connecting to the plasmasphere. Furthermore, coincident longitudinal variations exist in both the ionospheric enhancements and the plasmaspheric total electron content, especially during the solstice months. The coincidence may suggest essential plasma transport between the ionosphere and the plasmasphere. These facts support the idea that the plasmasphere provides extra plasma to the midlatitude ionosphere through downward plasma influx along the magnetic field lines to form the nighttime ionization enhancements when the sunlight is absent. Key Points Satellites reveal global pictures of nighttime enhancements in the midlatitude ionosphere at altitude of ∼350 and 850 km We find correlation of longitudinal dependence between the ionospheric enhancements and the plasmasphere The plasmasphere is suggested to feed and dominate the formation of the nighttime enhancements