•The analysis of spontaneous movements of preterm infants is essential because anomalous motion patterns can be a sign of neurological disorders.•A detection of anomalous motion patterns in the first weeks of child’s life is crucial to plan appropriate rehabilitative interventions.•We implement an interpretable pipeline based on machine learning and computer vision to characterize and classify infants’ motion from 2D video recordings.•Our procedure successfully discriminates normal and anomalous motion patterns with a maximum accuracy of 85.7%. Background and Objective: The analysis of spontaneous movements of preterm infants is important because anomalous motion patterns can be a sign of neurological disorders caused by lesions in the developing brain. A diagnosis in the first weeks of child’s life is crucial to plan timely and appropriate rehabilitative interventions. An accurate visual assessment of infants’ spontaneous movements requires highly specialized personnel, not always available, and it is operator dependent. Motion capture systems, markers and wearable sensors are commonly used for human motion analysis, but they can be cumbersome, limiting their use in the study of infants’ movements. Methods: In this paper we propose a computer-aided pipeline to characterize and classify infants’ motion from 2D video recordings. The final goal is detecting anomalous motion patterns. The implemented pipeline is based on computer vision and machine learning algorithms and includes a specific step to increase the interpretability of the results. Specifically, it can be summarized by the following steps: (i) body keypoints detection: we rely on a deep learning-based semantic features detector to localize the positions of meaningful landmark points on infants’ bodies; (ii) parameters extraction: starting from the trajectories of the detected landmark points, we extract quantitative parameters describing infants motion patterns; (iii) classification: we implement different classifiers (Support Vector Machines, Random Forest, fully connected Neural Network, Long Short Term Memory) that, starting from the motion parameters, classify between normal or abnormal motion patterns. Results: We tested the proposed pipeline on a dataset, recorded at the 40th gestational week, of 142 infants, 59 with evidence of neuromotor disorders according to a medical assessment carried out a posteriori. Our procedure successfully discriminates normal and anomalous motion patterns with a maximum accuracy of 85.7%. Conclusions: In conclusion, our pipeline has the potential to be adopted as a tool to support the early detection of abnormal motion patterns in preterm infants.