The thermal comfort of an enclosed room with air conditioner and air-distribution duct coupling can be studied, and the parameters of a split-fiber air conditioner can be optimized on the basis of studying the thermal comfort of various parts of the human body. In this paper, a room model with a distributed air conditioner was proposed. First, the rationality of the three thermal comfort characterization models of predict mean vote (PMV), predicted percentage of dissatisfied (PPD), and percentage of dissatisfied (PD) were verified through experiments and simulations. Then, the temperature and thermal comfort of various parts of the human body were explored when the air-distribution duct had different openings and different positions of the air outlet. The simulation results showed that compared with other situations, when the split-fiber air conditioner had three rows of holes (5-o’clock, 6-o’clock, 7-o’clock) and the air outlet was located in the middle of the right wall of the human body, the PMV, PPD, and PD of the measuring points around the human body fluctuated less, the indoor temperature field distribution fluctuated less, and there was no wind feeling around the human body, which can better meet the needs of human thermal comfort.