Display omitted •Zinc substitution was successfully achieved in Strontium Phosphosilicate.•Needle morphology of P63/m was observed to be retained with Zinc Substitution.•Optimised substitution levels were decided based on biocompatibility studies.•Antibacterial efficacy of parent material increased with respective levels of doping.•Apatite seeding was discovered in vitro that ultimately leads to bone mineralization. Synthesis of Pure phase and a series of Zinc substituted Strontium phosphosilicate was attempted in the present study and assessed structurally and biologically. The Sol-Gel technique ensured proper reactivity and apatite formation upon sintering. The acidic medium was more applicable for synthesis on account of the isoelectric point of silica and formation of secondary Sr2SiO4 phase, important and applicable in bioactivity apart from Sr2P2O7 as another secondary phase. Sintering formed agglomerated stable crystalline phases evident from diffraction further confirmed by infrared spectroscopy. The morphology showed minimal differences from the parent material increasing agglomeration upon the increment of dopant. Biological evaluations showed the successful formation of amorphous Calcium phosphate with two days of SBF immersion and proliferation of MG-63 Cells upon Zinc incorporation. The antibacterial properties were enhanced upon the incorporation of Zinc while Strontium phosphosilicate in the pure phase failed to produce favourable antibacterial activity.