There is an increasing need for diagnostic tools that can assess the crop nitrogen (N) nutrition status during the growth cycle. In addition to the leaf chlorophyll (Chl) content, we proposed here the use of the leaf content of polyphenolics (Phen) as a potential indicator of crop N status. Because of their absorption features in the visible and in the UV part of the spectrum, both Chl and Phen can be measured by rapid and non-destructive optical methods. Therefore, we used two leaf-clip devices, the Minolta SPAD-502 for Chl, and the Dualex for Phen. The latter is a prototype (patent pending) that measures the UV absorbance of the leaf epidermis, which is related to the leaf Phen content. Dynamics of Phen and Chl were measured on the last fully developed leaves of two winter wheat cultivars subjected to different levels of N availability, from tillering to flowering, in 2001, 2002 and 2003. Both Phen and Chl contents were found to increase along the leaf, starting from the ligula, regardless of the stage of development. Both variables were highly correlated with the N concentration of leaves. The average Chl content of the leaf increased, and the average Phen content decreased, with the increased application of N to the field, irrespective of the growth stage, the cultivar and the year of experiment. Therefore, both Phen and Chl can be considered as probes of the crop N nutrition status. Still, the relationship between Chl and the nitrogen nutrition index (NNI), used as a reference indicator of N deficiency, was influenced by the growth stage, whereas the year of experiment affected the relationship between Phen and the NNI. We also propose the use of the simple Chl/Phen ratio as an indicator of leaf N content at the canopy level, for future application in precision agriculture. This ratio would alleviate, at least partially, the problem of gradients along leaves, and would even accentuate the differences among levels of crop N deficiencies because of the Chl and Phen inverse dependence on the crop N nutrition status.