Display omitted •An integrated paper-based platform is proposed for the HSA concentration detection.•BCG reagent and HSA concentration is induced albumin-BCG complex for RGB detection.•HAS concentration of 36 real-world human samples are measured.•The results are in excellent agreement of R2 = 0.9912 versus official method. A rapid analytical platform composed of a paper-based chip (µPB-Chip) and a small analytic box is developed to determine the concentration of human serum albumin (HSA). In this study, a Bromocresol Green (BCG) reagent is dropped into the reaction area of the µPB-Chip, and the HSA concentration was derived from the R (red) value intensity of the albumin-BCG complex formed after a reaction process at 37 °C for 12 min. The effectiveness of the developed method is displayed using albumin concentrations ranging from 0 to 5 g/dL in HSA control samples. The measurement results acquired from 36 volunteer patient’s HSA samples are presented to be in good consistency with those obtained utilizing a conventional assay technique (R2 = 0.9912). Ultimately, the results show that the developed platform provides a convenient, economical and dependable method for HSA concentration detection.