252Cf neutron source has been widely used in biological effects of neutron radiation, neutron activation analysis and nuclear data acquisition. A seed irradiation device based on 252Cf fission neutron source was designed in this paper to study mutation breeding induced by neutron irradiation and biological effects of radiation. Based on the Monte Carlo method, neutron absorbed dose of pea seed samples in the designed irradiation region was simulated and calculated by MCNP. Results demonstrated that the relative standard deviation of the absorbed dose of pea seed samples in the three-layer partition is controlled lower than 10%, indicating the high dose uniformity in designed irradiation region. The experiment verified that the design of shield in this device meet the irradiation protection requirements.