Element concentrations were measured in multifloral honeys sampled from Central and Eastern Croatia. The mean levels of elements ranged from (µg/kg): Al 323–7228, Cu 103–1033, Cr 14.4–139, Fe 295–2336, Ni 122–523, Pb 9.65–154, Zn 442–2025. In all samples, As and Cd content were below the LOD values. Significant differences in the concentrations of Al, Cr, Cu, Fe, Pb and Zn ( p  < 0.01) were found in honeys from different locations within regions and within locations of each region. Also, significant differences in total element contents between the two regions were determined for Cr and Cu ( p  < 0.01). No significant differences were observed in total Al, Fe, Pb and Zn levels between regions. The highest Al, Cr, Cu, Fe and Zn concentrations were measured in Central Croatia, while Ni and Pb in Eastern Croatia. The results confirm the decisive influence of collection location on the composition of toxic and trace elements in honey.