•The ectoparasiticide treatment is enough to decrease otitis lesion caused by P. ovis.•Vectra 3D® could be used to control infestation by P. ovis in rabbits.•Spontaneous lesions remission can occur in otitis caused by P. ovis in rabbits. The main of this study was to evaluate the efficacy of dinotefuran, pyriproxyfen and permethrin-combination (Vectra 3D-CEVA) in the topical treatment of rabbits naturally infested with P. ovis. Adult New Zealand rabbits (n = 18) with plaques of crust in both ears were divided into three groups (one control and two treated). On day 0, the animals belonging to the treated groups received a single dose of a commercial ectoparasiticide formulation, recommended for use in dogs. The control group (G1) (n = 6) received no treatment, the treated group (G2) (n = 6) received one drop in each ear, and the remaining volume was applied along the back of the animal in the dorsal midline with the commercial formulation. In the other treated group (G3) (n = 6), animals received the same product used in G2, but all the volumes of 0.5 mL were applied only to the back of the animal (dorsal midline). On days 0, +7, +14, +21, +28 and +35, lesion scores and mites per gram (MPG) of each ear scab were evaluated. Statistical analysis was performed using the statistical software Bioestat 5.01. As the Shapiro-Wilk test determined that the data were nonparametrically distributed, the Kruskal–Wallis test was performed, followed by the Student-Newman–Keuls test, to determine the significance levels among the mean values of mite counts per gram of crusts compared to the three experimental groups. The efficacies for remission of lesion scores were 19.87 % on day +7 to 83.44 % on day +35 for G2 and 70.67 % on day +7 to 92.20 % on day +35 for G3. The efficacies obtained by evaluation of MPG were 100 % on day +7 to 99.86 % on day +35 for G2 and from 93.05 % on day +7 to 99.89 on day +35 for G3. The topical administration of the combination of dinotefuran (4.95 %), pyriproxifen (0.44 %) and permethrin (36 %) on the ears and back or only on the back in rabbits was shown to be effective in naturally controlling mite infestations by P. ovis.