The research on land use transformation has attracted more and more attention, and there are few research results on the eco-environmental effects of regional land use change from the perspective of land use function transformation. From the perspective of production-living-ecological land use function classification, this paper obtained land use change data of Shaanxi Province in 2000, 2010, and 2020, calculated the land use transfer matrix, ecological environment quality index, and ecological contribution rate of land use transformation, and quantitatively analyzed the temporal and spatial evolution of production-living-ecological spaces and the eco-environmental effects of land use structure transformation in Shaanxi Province. The results showed that the land use change in Shaanxi Province is mainly manifested in the continuous increase of ecological and living land area and the continuous decrease of production land area from 2000 to 2020. In the transformation of land use structure in Shaanxi Province, the biggest change in area is the mutual transformation between productive cultivated land and ecological grassland land. The occupation of ecological grassland land by other land types is an important factor leading to the decline of ecological environment quality. Overall, the ecological environment of Shaanxi Province has shown a trend of improvement in the past two decades. Quantitative research on the transformation characteristics of land use structure and its ecological environment effects in the past two decades is conducive to providing data support for the promotion of ecological civilization construction in Shaanxi Province.