E-viri
Recenzirano
Odprti dostop
-
Mahony, Jennifer; Frantzen, Cyril; Vinogradov, Evgeny; Sadovskaya, Irina; Theodorou, Ilias; Kelleher, Philip; Chapot‐Chartier, Marie‐Pierre; Cambillau, Christian; Holo, Helge; Sinderen, Douwe
Molecular microbiology, October 2020, 2020-10-00, 20201001, 2020-10, Letnik: 114, Številka: 4Journal Article
The biosynthetic machinery for cell wall polysaccharide (CWPS) production in lactococci is encoded by a large gene cluster, designated cwps. This locus displays considerable variation among lactococcal genomes, previously prompting a classification into three distinct genotypes (A–C). In the present study, the cwps loci of 107 lactococcal strains were compared, revealing the presence of a fourth cwps genotype (type D). Lactococcal CWPSs are comprised of two saccharidic structures: a peptidoglycan‐embedded rhamnan backbone polymer to which a surface‐exposed, poly/oligosaccharidic side‐chain is covalently linked. Chemical structures of the side‐chain of seven lactococcal strains were elucidated, highlighting their diverse and strain‐specific nature. Furthermore, a link between cwps genotype and chemical structure was derived based on the number of glycosyltransferase‐encoding genes in the cwps cluster and the presence of conserved genes encoding the presumed priming glycosyltransferase. This facilitates predictions of several structural features of lactococcal CWPSs including (a) whether the CWPS possesses short oligo/polysaccharide side‐chains, (b) the number of component monosaccharides in a given CWPS structure, (c) the order of monosaccharide incorporation into the repeating units of the side‐chain (for C‐type strains), (d) the presence of Galf and phosphodiester bonds in the side‐chain, and (e) the presence of glycerol phosphate substituents in the side‐chain. The chemical structures of cell wall polysaccharides (CWPS) of seven distinct lactococcal strains were elucidated. Comparative genome analysis of the gene clusters that encode these structures of 107 lactococcal strains has led to the identification of four distinct genotypes that correlates to distinct chemical structures. The combined genome and structural data allow predictions of characteristics of lactococcal CWPS based on sequence information, while a model for the biosynthetic pathway of the CWPS of the prototypical B‐type strain IL1403 is proposed.
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.