Background: Knowledge of the spatial trends of plant invasions in different habitats is essential for a better understanding of the process of these invasions. We examined the variation in invasive alien plant species (IAS) richness and composition at two spatial scales defined by elevation and habitat types (roadside, forest, and cultivated lands) in the Makawanpur district of Nepal. Following an elevation gradient ranging from 500 to 2,400 m asl along a mountain road, plant species cover was recorded within sample plots of size 10 m × 5 m. Systematic random sampling was adopted in every 100 m elevation intervals on three habitat types. Results: Altogether 18 invasive alien plants belonging to eight families were recorded within 60 plots, of which 14 species (representing 80%) were from tropical North and South America. The most common plants by their frequency were Ageratina adenophora, Chromolaena odorata, Bidens pilosa, Lantana camara, and Parthenium hysterophorus. We found a significant relationship between species composition and elevation in the study area. Low-elevation regions had a higher number of alien species as compared to high-elevation regions within different habitat types. Conclusions: The species richness and density of IAS were higher in the road site followed by the cultivated land and forest sites. This pattern occurred throughout the elevation range and habitats. IAS were found mostly in the open land with high sunlight availability. Information from such scientific assessment of invasive alien plants will assist in developing appropriate management plans in the Makawanpur district. KCI Citation Count: 0