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Bayliss, C.D; Peters, R.W; Cook, J.K.A; Reece, R.L; Howes, K; Binns, M.M; Boursnell, M.E.G
Archives of virology, 01/1991, Letnik: 120, Številka: 3/4Journal Article
The coding sequences of VP2 from a virulent strain, 52/70, of infectious bursal disease virus (IBDV) were excised from a cDNA clone and inserted into a fowlpox plasmid insertion vector. The resulting plasmid, pIBD 1, was used to construct a recombinant fowlpox virus, fpIBD 1, which expressed VP 2 as a beta-galactosidase fusion protein. Chickens vaccinated with fpIBD 1 at 1 and 14 days of age, were challenged at 28 days with either IBDV strain 52/70 or the highly virulent strain CS 89. These chickens were protected against mortality, but not against damage to the bursa of Fabricius. The protection achieved by the use of fpIBD 1 shows that VP2 is a host protective antigen.
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in: SICRIS
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