The present study investigated and compared the diagnostic accuracy of Dysphonia Severity Index (DSI) and Acoustic Voice Quality Index (AVQI) in Indian adults in the age range of 18–40years across different levels of dysphonia severity. Normophonic individuals (n=163) and individuals with dysphonia (n=134) were selected using purposive sampling in the age range of 18–40years. For DSI, Computerized Speech Lab 4500 and for AVQI, Praat 6.1.03 was used for recording. The results of receiver operating characteristics (ROC) analysis for DSI have revealed that DSI can discriminate the normophonic versus mild, mild versus moderate, and moderate versus severe dysphonic with cut-off values of 1.36, −1.83, and −4.07, respectively. The sensitivity and specificity reported for mild versus moderate and moderate versus severe are slightly lower for obtained threshold points. For AVQI v.02.03, ROC analysis revealed that high sensitivity and specificity cut-off points for normophonic versus mild, mild versus moderate, and moderate versus severe dysphonic are 2.50, 3.86, and 6.21, respectively. AVQI is a more effective technique than DSI for distinguishing between dysphonia severities levels, particularly when it comes to mild versus moderate and moderate versus severe, according to comparisons of AROC curves made using the DeLang method.