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Kawahara, Yumi; Manabe, Tomotaka; Matsumoto, Masaya; Kajiume, Teruyuki; Matsumoto, Masayasu; Yuge, Louis
PloS one, 07/2009, Letnik: 4, Številka: 7Journal Article
Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required. We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF. Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
