Garlic is valuable crop that is used not only for human consumption, but also in pharmaceutical, food and cosmetics industries. Indigenous cultivars have specific beneficial properties that could be permanently lost due to cultivation of new cultivars. Aim of this study was to established two indigenous garlic cultivars in vitro under influence of growth regulator and light. Garlic varieties Slavonian winter (Croatia) and Vincek (Slovenia) were introduced on medium supplemented with two different concentrations of BAP (1 and 1,5 mg/L) and grown under two types of light (FLUO and LED). Results showed there were no significant influence of light type but concentration of growth regulator significantly influenced in vitro development of garlic microshoots of both investigated cultivars. The best treatment for Vincek garlic explants was nutrient medium supplemented with 1.5 mg/L BAP and LED lights, while for Slavonian winter garlic the best treatment showed to be FLUO light and 1,5 mg/L BAP.