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Kobayashi, T. (Kao Corp., Ichikai, Tochigi (Japan). Tochigi Research Inst.); Higaki, N; Yajima, N; Suzumatsu, A; Hagihara, H; Kawai, S; Ito, S
Bioscience, biotechnology, and biochemistry, 04/2001, Letnik: 65, Številka: 4Journal Article
An exopolygalacturonase exo-PGase; poly (1,4-α-D-galacturonide) galacturonohydrolase, EC 3.2.1.67 was found to be extracellularly produced by Bacillus sp. strain KSM-P443. The exo-PGase was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, through sequential column chromatographies. The enzyme had a molecular weight of approximately 45,000 and an isoelectric point of pH 5.8. The N-terminal sequence was Ser-Met-Gln-Lys-Ile-Lys-Asp-Glu-Ile-Leu-Lys-Thr-Leu-Lys-Val-Pro-Val-Phe and had no sequence similarity to those of other petinolytic enzymes reported to date. Maximum activity toward polygalacturonic acid (PGA) was observed at 60°C and at pH 7.0 in 100 mM Tris-HCl buffer without requiring any metal ions. When the chain length of oligogalacturonic acids increased, the apparent Km for them decreased, but the kcat values increased. This is the first bacterial exo-PGase that releases exclusively mono-galacturonic acid from PGA, di-, tri-, tetra-, and penta-galacturonic acids.
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