Display omitted •Synthesis of the new MIP based on poly(1-VI-co-TMPTMA).•Excellent adsorbent material for analytical selective extraction of AVM and MBM.•Efficient PT-MIP-SPE coupled HPLC-UV method was developed.•PT-MIP-SPE is a simple, inexpensive, versatile, and easy to perform technique.•PT-MIP-SPE was used in extraction of AVM and MBM of mineral water and grape juice. A simple HPLC method was developed for the determination of abamectin (ABA), eprinomectin (EPR), and moxidectin (MOX). Pipette-tip molecularly imprinted polymer solid-phase extraction (PT–MIP–SPE) using poly(1-vinylimidazole-co-trimethylolpropane trimethacrylate) as a selective adsorbent material was studied in detail, including the washing solvent, type and volume of eluent, pH, quantity of adsorbent material and sample volume. The performance criteria for linearity, sensitivity, precision, accuracy, recovery, robustness and stability have been assessed and were within the recommended guidelines. The mean extraction recoveries/relative standard deviation for ABA 1b, EPR, ABA 1a and MOX were 98.77 ± 3.82%, 88.19 ± 2.57%, 110.54 ± 1.52% and 100.42 ± 0.59%, respectively. Finally, the results proved that PT–MIP–SPE coupled to HPLC–UV is an economical, simple and easy-to-perform technique, and presented a high potential for extraction of macrocyclic lactones in mineral water and grape and juice samples.