The quest to improve the quality of nuclear data, such as half-lives, transition yields, and reaction cross-sections, is a shared endeavor among various areas of nuclear science. V is a vanadium isotope for which experimental data on neutron reaction cross-sections is needed. However, traditional isotope production techniques cannot produce V with high enough isotopic purity for some of these measurements. "Isotope harvesting" at the Facility for Rare Isotope Beams (FRIB) is a new isotope production technique that could potentially yield V with the necessary purity for such studies. In this case, Cr would be collected and allowed to generate V that can be separated from undecayed Cr to yield highly pure V. Thus, any protocol for producing pure V via isotope harvesting would involve utilizing a separation technique that can effectively separate Cr and V. In this study, the radiotracers Cr and V were used to develop possible radiochemical separation methodologies, which can be translated to obtain high purity V via this novel isotope production method. The developed protocols utilize either ion exchange or extraction chromatographic resins. Separations of Cr and V with AG 1-X8 anion exchange resin respectively resulted in recoveries of 95.6(26)% and 96.2(12)% with radionuclidic purities of 92(2)% and 99(1)%. An even more effective Cr and V separation was obtained with an extraction chromatographic resin (TRU resin) and 10 M HNO loading solution. Here, Cr and V respectively had recoveries of 94.1(28)% and 96.2(13)% with high radionuclidic purities (100(2)% and 100(1)%) in small volumes (8.81(8) mL and 5.39(16) mL). This study suggests that, to maximize the yield and isotopic purity of V, the best production protocol would involve utilizing two separations with TRU resin and 10 M HNO to isolate Cr and purify the generated V.