Human colorectal cancer is the third most common cancer around the world. Colorectal cancer has various risk factors, but current works have bolded a significant activity for the microbiota of the human colon in the development of this disease. Bacterial biofilm has been mediated to non-malignant pathologies like inflammatory bowel disease but has not been fully documented in the setting of colorectal cancer. The investigation has currently found that bacterial biofilm is mediated to colon cancer in the human and linked to the location of human cancer, with almost all right-sided adenomas of colon cancers possessing bacterial biofilm, whilst left-sided cancer is rarely biofilm positive. The profound comprehension of the changes in colorectal cancer can provide interesting novel concepts for anticancer treatments. In this review, we will summarize and examine the new knowledge about the links between colorectal cancer and bacterial biofilm. •Colorectal cancer is the third common cancer in males and the second common cancer in females.•Various risk factors proposed for colorectal cancer.•The investigation has found that biofilm is mediated to colon cancer and linked to the location of cancer.•It has led to the characterization of putative oncogenic drivers of colorectal cancer, for instance, enterotoxigenic B. fragilis producing the BFT, E. coli having the pks encoding the genes need to form the genotoxinʼs colibactin, and F. nucleatum containing the FadA.•Identifying candidate pro-carcinogenic bacteria from colon mucosal biofilms can enable earlier screening to predict patients at risk of developing colorectal cancer and allow the application of interventions to disrupt the progression of colon carcinogenesis.