Exploring new biomass sources for nanocellulose (NC) extraction is crucial in elevating the economic value of readily available renewable resources. This study compares NC extracted from acai (Euterpe oleracea) bagasse using different methods: mixed acid hydrolysis, 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO) mediation, and ammonium persulfate (APS) oxidations. A comprehensive analysis investigates the impact of each treatment on the physical-chemical properties of the nanoparticles, including chemical structure, crystallinity, morphology, and thermal and suspension stability. NCs obtained through mixed acid hydrolysis exhibit the highest crystallinity (62 %) and low sulfate groups on their surfaces. Consequently, they demonstrate excellent thermal stability but poor colloidal stability in water. Oxidized NCs undergo chemical modification, converting alcoholic groups into carboxyl, resulting in NCs with zeta potentials ranging between −25.30 ± 0.81 and − 27.49 ± 1.07 mV. APS oxidation produces nanoparticles with superior thermal stability compared to TEMPO oxidation. Atomic Force Microscopy (AFM) images reveal that all nanocelluloses share characteristics of nanofibers (CNFs). This comprehensive characterization highlights the potential of acai bagasse for yielding high-added-value bioproducts suitable for versatile applications. Display omitted •Nanocellulose from acai bagasse was extracted by hydrolysis and oxidative methods.•APS and TEMPO oxidations produced distinct types of ionic charged nanocelluloses.•Mixed acid hydrolysis produced nanocellulose with better thermal stability.•All nanocelluloses from acai bagasse had nanofiber's characteristics.