A lake sediment core spanning 9900 years, collected from a small lake on western Victoria Island, NWT, Canada, provides a high-resolution record of diatom community dynamics over the Holocene. Ten radiocarbon dates and 210Pb dating provided the core chronology. Loss-on-ignition (LOI), magnetic susceptibility, biogenic silica content, and diatom concentrations provided information on changes in the sedimentary environment. LOI gradually increased over the Holocene whereas magnetic susceptibility showed an inverse trend. Biogenic silica content showed three distinct peaks spaced approximately 3000 years apart. Major shifts in diatom assemblages occurred at 8100–8000, 5800–5700, and 3800–3500calyrBP. There is evidence of diatom community response to centennial-scale variations such as the ‘Medieval Warm Period’ (∼1000–700calyrBP), ‘Little Ice Age’ (∼800–150calyrBP) and recent warming. Although recent changes in diatom community composition, productivity, and species richness are apparent they were surpassed at other periods throughout the Holocene. Variations of the taxa within the genera Staurosira, Pseudostaurosira, Fragilaria, and Staurosirella, usually combined into one genus in Arctic lake sediment studies, suggest these taxa provide useful insight into paleonvironmental questions.