Introduction: The pharmacists played an important role in the development of biochemistry as applied chemistry in Serbia. What is more, the first seven state chemists in Ser bia were pharmacists. State chemists performed the chemicaltoxicological analysis as well as some medical and biochemical ones. When it comes to the education of medical biochemists as health workers, the period after the beginning of the second half of the twentieth century should be taken into account because that is when the training of pharmaceutical staff of the Faculty of Pharmacy, University of Belgrade, begins on the territory of Serbia. This paper presents the development of medical biochemistry through the development of curriculum, personnel and literature since the foundation of the Faculty of Pharmacy in Serbia until today. Objective: The aim of this paper is to present the historical development of biochemistry at the Faculty of Pharmacy, University of Belgrade, through analysis of three indicators: undergraduate and postgraduate education of medical biochemists, teaching literature and professional associations and trade associations. Method: The method of direct data was applied in this paper. Also, desktop analysis was used for analyzing of secondary data, regulations, curricula, documents and bibliographic material. Desktop research was conducted and based on the following sources: Archives of the University of Belgrade- Faculty of Pharmacy, Museum of the History of Pharmacy at the University of Belgrade-Faculty of Pharmacy, the Society of Medical Biochemists of Serbia and the Serbian Chamber of Biochemists. Results and conclusion: The curricula, the Bologna process of improving education, the expansion of the range of subjects, the number of students, professional literature for teaching biochemistry, as well as professional associations and trade associations are presented through the results. Uvod: Značajnu ulogu u razvoju biohemije kao primenjene hemije dali su farmaceuti, pa su čak prvih sedam državnih hemičara u Srbiji bili apotekari. Državni hemičar obavljao je hemijsko-toksikološke analize kao i neke medicinsko-bio- hemijske analize. O školovanju medicinskih biohemičara kao zdravstvenih radnika može se govoriti od druge polovine XX veka, kada na teritoriji Srbije počinje školovanje farmaceutskog kadra na Farmaceutskom fakultetu u Beogradu. U radu je prikazan razvoj medicinske biohemije kroz razvoj nastavnih planova i programa, stručnog kadra i literature na Farma­ceutskom fakultetu u Beogradu od njegovog osnivanja do danas. Cilj: Cilj rada je da se prikaže istorijski razvoj biohemije na Farmaceutskom fakultetu Univerziteta u Beogradu analizom tri indikatora: dodiplomskog i poslediplomskog obrazovanja medicinskih biohemičara; nastavne literature i stručnih udruženja i strukovnih asocijacija. Metode: U radu je primenjena metoda direktnih podataka i, gde je bilo potrebno, korišćena je desktop analiza pomoću koje su analizirani sekundarni podaci, uredbe, nastavni planovi i programi, dokumenta i bibliografska grada. Desktop istraživanje je realizovano na osnovu slededh izvora: Arhive Univerziteta u Beogradu - Farmaceutskog fakulteta, Muzeja za istoriju farmacije Univerziteta u Beogradu - Farmaceut­skog fakulteta, Društva medicinskih biohemičara Srbije i Komore biohemičara Srbije. Rezultati i zaključak: Kroz rezultate prikazani su nastavni planovi i programi, Bolonjski proces unapređenja nastave, širenje opsega predmeta, broj studenata, stručna literatura za nastavu iz biohemije i stručna udruženja i strukovne asocijacije.