Pollen, chironomid, and ostracode records from a lake located at alpine treeline provide regional paleoclimate reconstructions from the southwest Yukon Territory, Canada. The pollen spectra indicate herbaceous tundra existed on the landscape from 13.6–11 ka followed by birch shrub tundra until 10 ka. Although Picea pollen dominated the assemblages after 10 ka, low pollen accumulation rates and Picea percentages indicate minimal treeline movement through the Holocene. Chironomid accumulation rates provide evidence of millennial-scale climate variability, and the chironomid community responded to rapid climate changes. Ostracodes were found in the late glacial and early Holocene, but disappeared due to chemical changes of the lake associated with changes in vegetation on the landscape. Inferred mean July air temperature, total annual precipitation, and water depth indicate a long-term cooling with increasing moisture from the late glacial through the Holocene. During the Younger Dryas (12.9–11.2 ka), cold and dry conditions prevailed. The early and mid-Holocene were warm and dry, with cool, wet conditions after 4 ka, and warm, dry conditions since the end of the Little Ice Age.