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Bode, Edna; Brachmann, Alexander O.; Kegler, Carsten; Simsek, Rukayye; Dauth, Christina; Zhou, Qiuqin; Kaiser, Marcel; Klemmt, Petra; Bode, Helge B.
Chembiochem : a European journal of chemical biology, May 4, 2015, Letnik: 16, Številka: 7Journal Article
Exchange of the native promoter to the arabinose‐inducible promoter PBAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the “on‐demand” production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel “mevalagmapeptides” and the highly toxic xenorhabdins were identified by this approach. Sweet induction: The promoters of several gene clusters involved in natural product biosynthesis were exchanged to the arabinose‐inducible PBAD promoter in entomopathogenic bacteria to result in “knock‐out” (without inducer) or tenfold overproduction (with the arabinose inducer), as shown for xenoamicin A (white crystals) and other compounds.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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