Water contamination is regarded as one of the most important concerns in the last decade and its crucial to develop an efficient and productive technique to remediate organic pollutants from waters. Photocatalytic degradation processesare advanced oxidation procedure and a promising technique for the degradation of organic pollutants. In this study, we fabricated Ag2S/Zn3V2O8 nanocomposite (AS/ZVO NCs) for the degradation of rifampicin (RIF) by ultrasonicated co-precipitation strategy. The NCs was optimally synthesized with varying deposition concentrations, among which 5%-Ag2S/Zn3V2O8 (5%-AS/ZVO) exhibited superior photocatalytic activity (95.5%) under visible light irradiation. Moreover, the experiments were conducted at diverse operational parameters such as different NCs dosage, different concentration of RIF, pH, and ions, to investigate the impact on the photocatalytic degradation of RIF. Notably, XRD and XPS results of used 5%-AS/ZVO NCs demonstrated commendable stability and possess sustained integrity in structure after six consecutive cycle tests. The scavenging study and radical trapping experiments confirmed the formation of •OH and O2•- during photocatalytic reaction. The mechanism of photocatalytic degradation of RIF by 5%-AS/ZVO NCs was proposed and the pathway was predicted based on the GC-MS results. The current study provides a comprehensive understanding for designing efficient photocatalysts for the environmental remediation applications. •Ag2S/Zn3V2O8 was prepared for effective photodegradation of rifampicin.••OH and O2•- were primarily involved in photocatalytic reactions.•NCs exhibited excellent photocatalytic stability and structural integrity.•Investigation of intermediate product using GC-MS•The toxicity of intermediate product assessed using ECOSAR program.