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Longobardi, F.; Ventrella, A.; Bianco, A.; Catucci, L.; Cafagna, I.; Gallo, V.; Mastrorilli, P.; Agostiano, A.
Food chemistry, 2013-Dec-01, Letnik: 141, Številka: 3Journal Article
•Geographic origin of sweet cherries was characterised by NMR and statistics tools.•1H NMR was used in a nontargeted fingerprinting approach.•PLS-DA and LDA gave the best classification and prediction performances.•Different strategies for variable number reduction were tested.•External validation procedures were applied on the classification models. In this study, non-targeted 1H NMR fingerprinting was used in combination with multivariate statistical techniques for the classification of Italian sweet cherries based on their different geographical origins (Emilia Romagna and Puglia). As classification techniques, Soft Independent Modelling of Class Analogy (SIMCA), Partial Least Squares Discriminant Analysis (PLS-DA), and Linear Discriminant Analysis (LDA) were carried out and the results were compared. For LDA, before performing a refined selection of the number/combination of variables, two different strategies for a preliminary reduction of the variable number were tested. The best average recognition and CV prediction abilities (both 100.0%) were obtained for all the LDA models, although PLS-DA also showed remarkable performances (94.6%). All the statistical models were validated by observing the prediction abilities with respect to an external set of cherry samples. The best result (94.9%) was obtained with LDA by performing a best subset selection procedure on a set of 30 principal components previously selected by a stepwise decorrelation. The metabolites that mostly contributed to the classification performances of such LDA model, were found to be malate, glucose, fructose, glutamine and succinate.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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