Increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time RT-PCR assay

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Increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time RT-PCR assay

Pang, Xiaoli; Cao, Min; Zhang, Min; Lee, Bonita

Journal of virological methods, 03/2011, Letnik: 172, Številka: 1

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The real-time TaqMan RT-PCR assay ( Pang et al., 2004) did not detect 14 clinical samples with rotavirus G2 genotype. Three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to G2P4, G2P8, G3P4, G9P4, G8 and G12 sequences. An additional forward primer was designed and included in a modified assay to test the 14 clinical samples and 12 samples with known rotavirus G and P genotypes. The modified assay has improved significantly the sensitivity for specific rotavirus strains without affecting the detection of other genotypes, creating a molecular assay with broad detection of various genotypes of group A rotaviruses.

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Pang, Xiaoli | Cao, Min | Zhang, Min | Lee, Bonita

Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.

Leto	Faktor vpliva		Izdaja		Kategorija		Razvrstitev
Leto	JCR	SNIP	JCR	SNIP	JCR	SNIP	JCR	SNIP

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