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Rudnick, Paul A; Niles, Richard K; Vega-Montoto, Lorenzo J; Mani, D R; Abbatiello, Susan E; Tegeler, Tony J; Tempst, Paul; Gibson, Bradford W; Cusack, Michael P; Buck, Charles; Hall, Steven C; Neubert, Thomas A; Mesri, Mehdi; Tabb, David L; Johansen, Eric B; Ransohoff, David; Addona, Terri A; Bunk, David M; Hiltke, Tara; Zhao, Lei; Schilling, Birgit; Regnier, Fred E; Rodriguez, Henry; Liebler, Daniel C; Wahlander, Åsa; Ham, Amy-Joan L; Dodder, Nathan G; Spiegelman, Clifford H; Carr, Steven A; Li, Jing; Allen, Simon; Variyath, Asokan M; Waldemarson, Sofia; Smith, Derek; Jackson, Angela; Borchers, Christoph H; Anderson, N Leigh; Wang, Mu; Keshishian, Hasmik; Fisher, Susan J; Held, Jason M; Pulsipher, Trenton C; Blackman, Ronald K; Skates, Steven J; Whiteaker, Jeffrey R; Zimmerman, Lisa J; Cardasis, Helene L; Kinsinger, Christopher R; Paulovich, Amanda G
Nature biotechnology, 07/2009, Letnik: 27, Številka: 7Journal Article
Verification of candidate biomarkers relies upon specific, quantitative assays optimized for selective detection of target proteins, and is increasingly viewed as a critical step in the discovery pipeline that bridges unbiased biomarker discovery to preclinical validation. Although individual laboratories have demonstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can quantify candidate protein biomarkers in plasma, reproducibility and transferability of these assays between laboratories have not been demonstrated. We describe a multilaboratory study to assess reproducibility, recovery, linear dynamic range and limits of detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC. Using common materials and standardized protocols, we demonstrate that these assays can be highly reproducible within and across laboratories and instrument platforms, and are sensitive to low mug/ml protein concentrations in unfractionated plasma. We provide data and benchmarks against which individual laboratories can compare their performance and evaluate new technologies for biomarker verification in plasma.
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Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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