In , two genes ( and ) have been found to be associated with the invasion of the amniotic cavity, and a single gene ( ) to be associated with intra-amniotic infections and a high risk of preterm birth. The syntopic presence of spp. in the same patient has been shown to correlate with the absence of in . The aim of our study was to investigate the presence of , , and genes in two groups of isolates collected from symptomatic and asymptomatic male and non-pregnant female patients attending an Outpatients Centre. Group A consisted of 26 isolates from patients with only confirmed; group B consisted of 24 isolates from patients with spp. as the only co-infection. We extracted DNA from all isolates and analysed the samples for the presence of , , and in a qPCR assay. Additionally, we determined their cytotoxicity against HeLa cells. We confirmed the presence of the gene in 85% of group A isolates and in 100% of group B isolates; was detected in 46% of the samples in both groups, whereas was found in 73% of group A and 79% of group B isolates, respectively. It was shown that co-colonisation with spp. in the same patient had no effect on the presence of in the respective isolate. We did not observe any cytotoxic effect of the investigated isolates on human cells, regardless of the presence or absence of the investigated genes.