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Hibbs, Margaret L.; Selvaraj, Periasamy; Carpén, Olli; Springer, Timothy A.; Kuster, Helmut; Jouvin, Marie-Hélène E.; Kinet, Jean-Pierre
Science (American Association for the Advancement of Science), 12/1989, Letnik: 246, Številka: 4937Journal Article
Granulocyte and natural killer (NK) cell Fc receptors for immunoglobulin G (CD16) differ in only a few amino acids, yet have phosphatidylinositol glycan (PIG) or polypeptide membrane anchors, respectively. Mutagenesis shows that anchoring is regulated by a serine residue near the PIG anchor attachment site in the extracellular domain. The NK cell isoform was not expressed on the surface of COS cells unless cotransfected with a subunit that was expressed in NK cells and that was identical to the $\gamma $ subunit of the high affinity IgE Fc receptor (Fc$\epsilon $RI). However, the CD16 sequence and not expression of the $\gamma $ subunit is dominant in regulating PIG reanchoring.
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