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Performance of the extended use of the FilmArray® BCID panel kit for bronchoalveolar lavage analysisSansot, Maxime; Fradin, Eugénie; Chenouard, Rachel; Kempf, Marie; Kouatchet, Achille; Lasocki, Sigismond; Lemarié, Carole; Eveillard, Matthieu; Pailhoriès, Hélène
Molecular biology reports, 1/6, Letnik: 46, Številka: 3Journal Article
Ventilator-associated pneumonia (VAP) are responsible for an increase in morbidity, mortality, and prolonged hospital stay. A multiplex PCR kit such as the FilmArray ® BCID panel could allow early adaptation of antimicrobial therapy, which is crucial for clinical outcomes. The purpose of this study was to test the performances of FilmArray ® BCID panel for the detection of bacteria producing VAP. We tested the FilmArray ® BCID panel on 50 bronchoalveolar lavages (BALs), from patients hospitalized in two intensive care units at the Angers university hospital, compared to the conventional culture-based method. The sensitivity and the specificity of the FilmArray ® BCID panel were 67.2% and 98.9% respectively. They were 88.6% and 98.3% respectively when considering BALs with a positive culture > 10 4 CFU/mL, and 94.7% and 99.6% respectively if considering BALs with a positive direct examination. This study underlines the good performance of the FilmArray ® BCID panel for BAL fluid analysis. In case of positive direct examination, this test allows reliable results that can be obtained at an early stage, facilitating the early adaptation of antimicrobial therapy.
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