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Kol, Stefan; Ley, Daniel; Wulff, Tune; Decker, Marianne; Arnsdorf, Johnny; Schoffelen, Sanne; Hansen, Anders Holmgaard; Jensen, Tanja Lyholm; Gutierrez, Jahir M; Chiang, Austin W T; Masson, Helen O; Palsson, Bernhard O; Voldborg, Bjørn G; Pedersen, Lasse Ebdrup; Kildegaard, Helene Faustrup; Lee, Gyun Min; Lewis, Nathan E
Nature communications, 04/2020, Letnik: 11, Številka: 1Journal Article
Host cell proteins (HCPs) are process-related impurities generated during biotherapeutic protein production. HCPs can be problematic if they pose a significant metabolic demand, degrade product quality, or contaminate the final product. Here, we present an effort to create a "clean" Chinese hamster ovary (CHO) cell by disrupting multiple genes to eliminate HCPs. Using a model of CHO cell protein secretion, we predict that the elimination of unnecessary HCPs could have a non-negligible impact on protein production. We analyze the HCP content of 6-protein, 11-protein, and 14-protein knockout clones. These cell lines exhibit a substantial reduction in total HCP content (40%-70%). We also observe higher productivity and improved growth characteristics in specific clones. The reduced HCP content facilitates purification of a monoclonal antibody. Thus, substantial improvements can be made in protein titer and purity through large-scale HCP deletion, providing an avenue to increased quality and affordability of high-value biopharmaceuticals.
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Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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